Document Detail


Expression of galectin-3 in cells exposed to stress-roles of jun and NF-kappaB.
MedLine Citation:
PMID:  10878445     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND/AIMS: Galectin-3 is an interesting intracellular lectin that appears to be involved in numerous physiological processes. We have analyzed expression of galectin-3 in glioblastoma cells exposed to heat-shock, alkylating agents, UV-C radiation and subculturing (trypsinization). METHODS: Protein levels of galectin-3 were measured by western-blot analysis using M3/38 monoclonal antibody. The involvement of transcription factors NF-kappaB and Jun in the induction of galectin-3 was addressed using specific inhibitor of NF-kappaB (zL(3)-vs) and antisense-jun oligonucleotides. RESULTS: Exposure of cells to heat-shock or subculturing (trypsinization) decreased levels of galectin-3 to approximately 50%. Alkylating damage and UV-C irradiation caused an increase in the expression of galectin-3. Both inhibition of Jun by antisense-jun oligonucleotides, and inhibition of NF-kappaB by specific proteasomal inhibitor attenuated the induction of galectin-3 by UV-light, but with somewhat different kinetics. CONCLUSIONS: We have found that different forms of cellular stress have different effects on the expression of galectin-3. Heat-shock and subculturing decrease, while alkylating agents and UV-light increase galectin 3. NF-kappaB and Jun were shown to be involved in the induction of galectin-3 by UV-light, which is a first demonstration that these transcriptional factors are involved in the regulation of galectin-3 expression.
Authors:
J Dumic; G Lauc; M Flögel
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology     Volume:  10     ISSN:  1015-8987     ISO Abbreviation:  Cell. Physiol. Biochem.     Publication Date:  2000  
Date Detail:
Created Date:  2000-10-19     Completed Date:  2000-10-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9113221     Medline TA:  Cell Physiol Biochem     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  149-58     Citation Subset:  IM    
Copyright Information:
Copyright 2000 S. Karger AG, Basel.
Affiliation:
Department of Biochemistry and Molecular Biology, Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kovavica 1, 10000 Zagreb, Croatia.
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MeSH Terms
Descriptor/Qualifier:
Alkylating Agents / pharmacology
Antigens, Differentiation / genetics*
Galectin 3
Gene Expression Regulation
Heat-Shock Response
Humans
NF-kappa B / metabolism*
Proto-Oncogene Proteins c-jun / metabolism*
Trypsin / pharmacology
Tumor Cells, Cultured
Ultraviolet Rays
Chemical
Reg. No./Substance:
0/Alkylating Agents; 0/Antigens, Differentiation; 0/Galectin 3; 0/NF-kappa B; 0/Proto-Oncogene Proteins c-jun; EC 3.4.21.4/Trypsin

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