Document Detail

Expression of the fusogenic HERV-FRD Env glycoprotein (syncytin 2) in human placenta is restricted to villous cytotrophoblastic cells.
MedLine Citation:
PMID:  16714059     Owner:  NLM     Status:  MEDLINE    
Recently, the expression of a human endogenous retrovirus HERV-FRD, able to encode a fusogenic envelope protein (syncytin 2), has been observed in human placenta. The aim of the present study was to localize the expression of syncytin 2 in first trimester placenta. In addition, we investigated the presence of HERV-FRD transcripts during the in vitro differentiation of isolated villous and extravillous trophoblastic cells from first trimester chorionic villi. Using a monoclonal antibody specifically raised against the HERV-FRD Env protein, syncytin 2 was immunolocalized only in the villous trophoblast of the chorionic villi, at the level of cytotrophoblastic cells. Interestingly, immunostaining was not observed in all cells but only in some of them, and was detected, more frequently, at the membrane level at the interface between the cytotrophoblastic cells and syncytiotrophoblast. Labeling was observed neither in the syncytiotrophoblast nor in the mesenchymal core of the villi nor in the extravillous trophoblast. In vitro detection of HERV-FRD transcripts was restricted to villous trophoblastic cells and decreased significantly with time in culture. These results suggest that syncytin 2 might play a role in human trophoblastic cell fusion.
A Malassiné; S Blaise; K Handschuh; H Lalucque; A Dupressoir; D Evain-Brion; T Heidmann
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Publication Detail:
Type:  Journal Article     Date:  2006-05-22
Journal Detail:
Title:  Placenta     Volume:  28     ISSN:  0143-4004     ISO Abbreviation:  Placenta     Publication Date:    2007 Feb-Mar
Date Detail:
Created Date:  2006-12-22     Completed Date:  2007-03-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8006349     Medline TA:  Placenta     Country:  England    
Other Details:
Languages:  eng     Pagination:  185-91     Citation Subset:  IM    
INSERM, U767, 4 avenue de l'Observatoire, 75006 Paris, France; Université Paris Descartes, Faculté des Sciences Pharmaceutiques et Biologiques, 4 avenue de l'Observatoire, 75006 Paris, France.
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MeSH Terms
Cells, Cultured
Endogenous Retroviruses / metabolism*
Gene Products, env / metabolism*
Placenta / metabolism*
Pregnancy / metabolism*
Pregnancy Proteins / metabolism*
Pregnancy Trimester, First / metabolism
Reg. No./Substance:
0/Gene Products, env; 0/Pregnancy Proteins; 0/syncytin 2 protein, human

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