Document Detail

Expression of a fungal hydrophobin in the Saccharomyces cerevisiae cell wall: effect on cell surface properties and immobilization.
MedLine Citation:
PMID:  12089019     Owner:  NLM     Status:  MEDLINE    
The aim of this work was to modify the cell surface properties of Saccharomyces cerevisiae by expression of the HFBI hydrophobin of the filamentous fungus Trichoderma reesei on the yeast cell surface. The second aim was to study the immobilization capacity of the modified cells. Fusion to the Flo1p flocculin was used to target the HFBI moiety to the cell wall. Determination of cell surface characteristics with contact angle and zeta potential measurements indicated that HFBI-producing cells are more apolar and slightly less negatively charged than the parent cells. Adsorption of the yeast cells to different commercial supports was studied. A twofold increase in the binding affinity of the hydrophobin-producing yeast to hydrophobic silicone-based materials was observed, while no improvement in the interaction with hydrophilic carriers could be seen compared to that of the parent cells. Hydrophobic interactions between the yeast cells and the support are suggested to play a major role in attachment. Also, a slight increase in the initial adsorption rate of the hydrophobin yeast was observed. Furthermore, due to the engineered cell surface, hydrophobin-producing yeast cells were efficiently separated in an aqueous two-phase system by using a nonionic polyoxyethylene detergent, C(12-18)EO(5).
Tiina Nakari-Setälä; Joana Azeredo; Mariana Henriques; Rosário Oliveira; José Teixeira; Markus Linder; Merja Penttilä
Related Documents :
6769679 - Cell surface immunoglobulin mu and gamma chains of human lymphoid cells are of higher a...
22282659 - Ribosome biogenesis and control of cell proliferation: p53 is not alone.
6348049 - Identification of the cohesion molecule, contact sites b, of dictyostelium discoideum.
22545049 - Overexpression of the orotate phosphoribosyl-transferase gene enhances the effect of 5-...
6573539 - Analysis of lectin receptors on rat embryo fibroblasts transformed by adenovirus 2.
457749 - Studies on the function of cell surface glycoproteins. ii. possible role of surface gly...
22946929 - 2-deoxy-d-glucose and 6-aminonicotinamide-mediated nrf2 down regulation leads to radios...
10208729 - Proliferation indices in spontaneous canine lung cancer: proliferating cell nuclear ant...
6472319 - Dna repair, dna synthesis and cell cycle delay in human lymphoblastoid cells differenti...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  68     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2002 Jul 
Date Detail:
Created Date:  2002-06-28     Completed Date:  2002-07-29     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3385-91     Citation Subset:  IM    
VTT Biotechnology, PO Box 1500, FIN-02044 VTT, Finland.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Binding Sites
Cell Wall / metabolism*
Fungal Proteins / biosynthesis*,  metabolism
Hydrophobic and Hydrophilic Interactions
Mannose-Binding Lectins
Membrane Proteins / genetics
Recombinant Fusion Proteins / metabolism
Saccharomyces cerevisiae / metabolism*,  physiology
Saccharomyces cerevisiae Proteins*
Trichoderma / genetics
Reg. No./Substance:
0/FLO1 protein, S cerevisiae; 0/Fungal Proteins; 0/Mannose-Binding Lectins; 0/Membrane Proteins; 0/Recombinant Fusion Proteins; 0/Saccharomyces cerevisiae Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Technical-scale production of cyanophycin with recombinant strains of Escherichia coli.
Next Document:  Heterologous expression of the Lactococcus lactis bacteriocin, nisin, in a dairy Enterococcus strain...