Document Detail


Expression of a fungal hydrophobin in the Saccharomyces cerevisiae cell wall: effect on cell surface properties and immobilization.
MedLine Citation:
PMID:  12089019     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of this work was to modify the cell surface properties of Saccharomyces cerevisiae by expression of the HFBI hydrophobin of the filamentous fungus Trichoderma reesei on the yeast cell surface. The second aim was to study the immobilization capacity of the modified cells. Fusion to the Flo1p flocculin was used to target the HFBI moiety to the cell wall. Determination of cell surface characteristics with contact angle and zeta potential measurements indicated that HFBI-producing cells are more apolar and slightly less negatively charged than the parent cells. Adsorption of the yeast cells to different commercial supports was studied. A twofold increase in the binding affinity of the hydrophobin-producing yeast to hydrophobic silicone-based materials was observed, while no improvement in the interaction with hydrophilic carriers could be seen compared to that of the parent cells. Hydrophobic interactions between the yeast cells and the support are suggested to play a major role in attachment. Also, a slight increase in the initial adsorption rate of the hydrophobin yeast was observed. Furthermore, due to the engineered cell surface, hydrophobin-producing yeast cells were efficiently separated in an aqueous two-phase system by using a nonionic polyoxyethylene detergent, C(12-18)EO(5).
Authors:
Tiina Nakari-Setälä; Joana Azeredo; Mariana Henriques; Rosário Oliveira; José Teixeira; Markus Linder; Merja Penttilä
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  68     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2002 Jul 
Date Detail:
Created Date:  2002-06-28     Completed Date:  2002-07-29     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3385-91     Citation Subset:  IM    
Affiliation:
VTT Biotechnology, PO Box 1500, FIN-02044 VTT, Finland. tiina.nakari-setala@vtt.fi
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MeSH Terms
Descriptor/Qualifier:
Binding Sites
Cell Wall / metabolism*
Fungal Proteins / biosynthesis*,  metabolism
Hydrophobic and Hydrophilic Interactions
Mannose-Binding Lectins
Membrane Proteins / genetics
Recombinant Fusion Proteins / metabolism
Saccharomyces cerevisiae / metabolism*,  physiology
Saccharomyces cerevisiae Proteins*
Trichoderma / genetics
Chemical
Reg. No./Substance:
0/FLO1 protein, S cerevisiae; 0/Fungal Proteins; 0/Mannose-Binding Lectins; 0/Membrane Proteins; 0/Recombinant Fusion Proteins; 0/Saccharomyces cerevisiae Proteins
Comments/Corrections

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