Document Detail


Expression and function of sodium transporters in two opossum kidney cell clonal sublines.
MedLine Citation:
PMID:  12060589     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present study describes characteristic features of two clonal subpopulations of opossum kidney (OK) cells (OK(LC) and OK(HC)) that are functionally different but morphologically identical. The most impressive differences between OK(HC) and OK(LC) cells are the overexpression of Na+-K+-ATPase and type 3 Na+/H+ exchanger by the former, accompanied by an increased Na+-K+-ATPase activity (57.6 +/- 5.6 vs. 30.0 +/- 0.1 nmol P(i). mg protein(-1). min(-1)); the increased ability to translocate Na+ from the apical to the basolateral surface; and the increased Na+-dependent pH(i) recovery (0.254 +/- 0.016 vs. 0.094 +/- 0.011 pH units/s). Vmax values (in pH units/s) for Na+-dependent pHi recovery in OK(HC) cells (0.00521 +/- 0.0004) were twice (P < 0.05) those in OK(LC) (0.00202 +/- 0.0001), with similar Km values (in mM) for Na+ (OK(LC), 21.0 +/- 5.5; OK(HC), 14.0 +/- 5.6). In addition, we measured the activities of transporters (organic ions, alpha-methyl-D-glucoside, L-type amino acids, and Na+ and enzymes (adenylyl cyclase, aromatic L-amino acid decarboxylase, and catechol-O-methyltransferase). The cells were also characterized morphologically by optical and scanning electron microscopy and karyotyped. It is suggested that OK(LC) and OK(HC) cells constitute an interesting cell model for the study of renal epithelial physiology and pathophysiology, namely, hypertension.
Authors:
Pedro Gomes; Jing Xu; Paula Serrão; Sofia Dória; Pedro A Jose; Patrício Soares-da-Silva
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  American journal of physiology. Renal physiology     Volume:  283     ISSN:  1931-857X     ISO Abbreviation:  Am. J. Physiol. Renal Physiol.     Publication Date:  2002 Jul 
Date Detail:
Created Date:  2002-06-12     Completed Date:  2002-07-22     Revised Date:  2011-04-28    
Medline Journal Info:
Nlm Unique ID:  100901990     Medline TA:  Am J Physiol Renal Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  F73-85     Citation Subset:  IM    
Affiliation:
Institute of Pharmacology and Therapeutics, Faculty of Medicine, 4200-319 Porto, Portugal.
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MeSH Terms
Descriptor/Qualifier:
Amiloride / analogs & derivatives*,  pharmacology
Animals
Aromatic-L-Amino-Acid Decarboxylases / metabolism
Biological Transport / drug effects,  physiology
Catechol O-Methyltransferase / metabolism
Clone Cells
Diuretics / pharmacology
Epithelial Cells / chemistry,  metabolism,  ultrastructure
Hypertension, Renal / metabolism
Immunoblotting
Kidney / cytology*
LLC-PK1 Cells
Leucine / pharmacokinetics
Levodopa / pharmacokinetics
Methylglucosides / pharmacokinetics
Microscopy, Electron, Scanning
Opossums
Parathyroid Hormone / pharmacology
Sodium-Hydrogen Antiporter / analysis,  genetics*,  metabolism*
Sodium-Potassium-Exchanging ATPase / analysis,  genetics*,  metabolism*
Swine
p-Aminohippuric Acid / pharmacokinetics
Grant Support
ID/Acronym/Agency:
DK-39308/DK/NIDDK NIH HHS; HL-23081/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Diuretics; 0/Levodopa; 0/Methylglucosides; 0/Parathyroid Hormone; 0/Sodium-Hydrogen Antiporter; 0/sodium-hydrogen exchanger 3; 1154-25-2/ethylisopropylamiloride; 25360-07-0/methylglucoside; 2609-46-3/Amiloride; 61-78-9/p-Aminohippuric Acid; 61-90-5/Leucine; EC 2.1.1.6/Catechol O-Methyltransferase; EC 3.6.3.9/Sodium-Potassium-Exchanging ATPase; EC 4.1.1.28/Aromatic-L-Amino-Acid Decarboxylases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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