Document Detail

Expression and function of leptin and its receptor in dairy goat mammary gland.
MedLine Citation:
PMID:  20181296     Owner:  NLM     Status:  MEDLINE    
Leptin is an autocrine and paracrine factor which affects the development and function of mammary gland. The purpose of this study was to investigate the presence and regulatory effect of leptin in Chinese Guan Zhong dairy goat mammary gland from the virgin state to involution. The protein expression and localization of leptin and its long form receptor (OB-Rb) were detected by western blot and a confocal laser scanning microscope. Explants were cultured to detect the impacts of leptin on mammary gland, western blot was used to research leptin signal transduction pathway in pregnancy, lactation and involution. Leptin and amounts of OB-Rb protein were high throughout the virgin stage and at the beginning of pregnancy, lower at mid-pregnancy and throughout lactation, then higher at involution. Immunofluorescence performed with the anti-leptin and anti-leptin receptor antibody showed labelling located in adipose, epithelial cells, or extracellular matrix at different stages. The localization of leptin and OB-Rb revealed that leptin induced the expression of OB-Rb specifically and controlled the development and physiological function of the mammary gland by binding to OB-Rb. Leptin stimulated the proliferation and differentiation of ductal epithelial cells in pregnancy by JAK-MAPK signal pathway, enhanced the amount of beta-casein in cultured lactating mammary gland by JAK-STAT5 signal pathway, made the mammary duct disappear and induced apoptosis of mammary epithelial cells and mammary gland restitution by JAK-STAT3 signal pathway in involution. Overall, this study demonstrated the importance and complexity of leptin and OB-Rb during mammary gland development and provides a valuable resource for future research in this area.
Meng Li; Qingzhang Li; Xuejun Gao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-02-25
Journal Detail:
Title:  The Journal of dairy research     Volume:  77     ISSN:  1469-7629     ISO Abbreviation:  J. Dairy Res.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-04-12     Completed Date:  2010-07-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985125R     Medline TA:  J Dairy Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  213-9     Citation Subset:  IM    
The Key Laboratory of Dairy Science of Education Ministry, Northeast Agricultural University, Harbin, China.
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MeSH Terms
Adipocytes / metabolism
Animals, Domestic
Caseins / metabolism
Cell Differentiation
Cell Proliferation
Cells, Cultured
Epithelial Cells / cytology,  metabolism
Extracellular Matrix / metabolism
Janus Kinases / metabolism
Lactation / metabolism*
Leptin / metabolism*,  pharmacology
Mammary Glands, Animal / cytology,  drug effects,  metabolism*
Mitogen-Activated Protein Kinases / metabolism
Receptors, Leptin / metabolism
STAT Transcription Factors / metabolism
Signal Transduction
Reg. No./Substance:
0/Caseins; 0/Leptin; 0/Receptors, Leptin; 0/STAT Transcription Factors; EC Kinases; EC Protein Kinases

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