Document Detail


Expression and function of class B scavenger receptor type I on both apical and basolateral sides of the plasma membrane of polarized testicular Sertoli cells of the rat.
MedLine Citation:
PMID:  15206959     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Class B scavenger receptor type I (SR-BI), a multiligand membrane protein, exists in various organs and cell types. In the testis, SR-BI is expressed in two somatic cell types: Leydig cells and Sertoli cells. Unlike interstitially localized Leydig cells, Sertoli cells present within the seminiferous tubules keep contact with spermatogenic cells and form the tight junction to divide the seminiferous epithelium into the basal and adluminal compartments. In this study, the expression and function of SR-BI in rat Sertoli cells were examined with respect to dependency on the spermatogenic cycle, the plasma membrane polarity, and the pituitary hormone follicle-stimulating hormone (FSH). When the expression of SR-BI was histochemically examined with testis sections, both protein and mRNA were already present in Sertoli cells during the first-round spermatogenesis and continued to be detectable thereafter. The level of SR-BI mRNA expression in Sertoli cells was lower at spermatogenic stages I-VI than at other stages. SR-BI was present and functional (in mediating cellular incorporation of lipids of high density lipoprotein) at both the apical and basolateral surfaces of polarized Sertoli cells. Finally, SR-BI expression at both the protein and mRNA levels was stimulated by FSH in cultured Sertoli cells. These results indicate that SR-BI functions on both the apical and basolateral plasma membranes of Sertoli cells, and that SR-BI expression in Sertoli cells changes during the spermatogenic cycle and is stimulated, at least in cultures, by FSH.
Authors:
Akihisa Nakagawa; Kaz Nagaosa; Tomoe Hirose; Kayoko Tsuda; Kunio Hasegawa; Akiko Shiratsuchi; Yoshinobu Nakanishi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Development, growth & differentiation     Volume:  46     ISSN:  0012-1592     ISO Abbreviation:  Dev. Growth Differ.     Publication Date:  2004 Jun 
Date Detail:
Created Date:  2004-06-21     Completed Date:  2004-10-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0356504     Medline TA:  Dev Growth Differ     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  283-98     Citation Subset:  IM    
Affiliation:
Graduate School of Medical Science, Kanazawa University, Shizenken, Kakuma-machi, Kanazawa, Ishikawa 920-1192, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD36
Cell Line
Cell Membrane / metabolism*
Cell Polarity
Cells, Cultured
Follicle Stimulating Hormone / physiology
Gene Expression Regulation
Leydig Cells / physiology
Male
Rats
Receptors, Immunologic / biosynthesis,  genetics,  physiology*
Receptors, Scavenger
Scavenger Receptors, Class B
Sertoli Cells / physiology*
Spermatogenesis
Testis / cytology,  physiology
Tight Junctions / metabolism
Chemical
Reg. No./Substance:
0/Antigens, CD36; 0/Receptors, Immunologic; 0/Receptors, Scavenger; 0/Scarb1 protein, rat; 0/Scavenger Receptors, Class B; 9002-68-0/Follicle Stimulating Hormone

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