Document Detail


Expression and function of Toll-like receptors in human endometrial epithelial cell lines.
MedLine Citation:
PMID:  19959241     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In mammals, Toll-like receptors (TLRs) are the principal family of innate immune pattern recognition receptors (PRRs). The main function for TLRs is the detection of molecular patterns associated with invading pathogens. We investigated TLR expression and function in three established human endometrial epithelial cell lines, including hTERT-EEC, HEC-1B and Ishikawa cells, and clarified the application of these endometrial cell lines as in vitro models for studying TLR expression and function in the female reproductive tract. TLR gene expression was examined by RT-PCR and protein localization by immunohistochemistry. Our results showed that TLR expression in these cell lines is comparable to published literature on TLR expression in primary human endometrial tissue. TLR function was investigated by the detection of IL-6 and IL-8 production by ELISA in response to TLR2, TLR3, TLR5, TLR7 and TLR9 ligands. We found that hTERT-EEC cells were responsive to TLR5 ligand and HEC-1B cells respond to TLR3 and TLR5 ligands. In contrast, Ishikawa cells respond only to PMA/I which was used as a positive control for IL-8 production. Finally, we investigated the influence of flagellin as a TLR5 stimulant on TLR5 expression in these cell lines by QPCR. Our results showed that the endometrial cell lines showed a tendency for increased TLR5 expression in response to flagellin stimulation and in hTERT-EEC cells this tendency was statistically significant. These results suggest that hTERT-EEC, HEC-1B and Ishikawa cell lines can be used as in vitro models to investigate innate immune responses of endometrial cells in the female reproductive tract.
Authors:
Wedad Aboussahoud; Reza Aflatoonian; Chris Bruce; Sarah Elliott; Jon Ward; Sue Newton; Sabine Hombach-Klonisch; Thomas Klonisch; Alireza Fazeli
Publication Detail:
Type:  Journal Article     Date:  2009-12-02
Journal Detail:
Title:  Journal of reproductive immunology     Volume:  84     ISSN:  1872-7603     ISO Abbreviation:  J. Reprod. Immunol.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2010-02-01     Completed Date:  2010-04-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8001906     Medline TA:  J Reprod Immunol     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  41-51     Citation Subset:  IM    
Copyright Information:
2009 Elsevier Ireland Ltd. All rights reserved.
Affiliation:
Academic Unit of Reproductive and Developmental Medicine, The University of Sheffield, Sheffield, UK.
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MeSH Terms
Descriptor/Qualifier:
Cell Line
Endometrium / chemistry,  immunology*
Epithelial Cells / chemistry,  immunology
Female
Flagellin / immunology
Genital Diseases, Female / immunology*
Humans
Immunity, Innate
Interleukin-6 / immunology
Interleukin-8 / immunology
Toll-Like Receptors / biosynthesis,  chemistry,  physiology*
Chemical
Reg. No./Substance:
0/Interleukin-6; 0/Interleukin-8; 0/Toll-Like Receptors; 12777-81-0/Flagellin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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