Document Detail

Expression of fugu TIMP-3 and -4 genes in adult tissues and embryos.
MedLine Citation:
PMID:  16753323     Owner:  NLM     Status:  MEDLINE    
The tissue inhibitors of metalloproteinases (TIMPs) are involved in various processes of extra-cellular matrix (ECM) metabolism by inhibiting matrix metalloproteinases (MMPs). However, the fundamental information for these genes is little known in fish. Previously, we report cDNA cloning and gene expressions of two fugu (Takifugu rubripes) TIMP-2s. Here, we cloned cDNA of fugu TIMP-3 and performed an expression analysis of TIMP-3 and -4 mRNA in fugu adult tissues using a quantitative real-time PCR. The expression level of TIMP-3 mRNA was constitutive in all tissues, while TIMP-4 was significantly higher in the brain (P=0.05). Further, we performed a whole mount in situ hybridization in fugu embryos at different stages. In early stages, TIMP-3 mRNA was abundant in the somites and the caudal end of the notochord. At hatching larvae, the TIMP-3 mRNA was abundant in the pectoral fin, dorsal and ventral fin fold along the entire antero-posterior axis. TIMP-3 may be involved in axis elongation and somitogenesis. TIMP-4 mRNA was expressed in the tail bud, at the midbrain-hindbrain boundary and in the diencephalon from 72 to 104 hpf. This indicates TIMP-4 is highly expressed in the brain matrix in vivo.
Hiroshi Tsukamoto; Yoshihiro Yokoyama; Tohru Suzuki; Shoshi Mizuta; Reiji Yoshinaka
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Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2006-05-04
Journal Detail:
Title:  Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology     Volume:  144     ISSN:  1096-4959     ISO Abbreviation:  Comp. Biochem. Physiol. B, Biochem. Mol. Biol.     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-07-03     Completed Date:  2006-09-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9516061     Medline TA:  Comp Biochem Physiol B Biochem Mol Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  395-403     Citation Subset:  IM    
Laboratory of Food Chemistry, Department of Marine Bioscience, Faculty of Biotechnology, Fukui Prefectural University, Obama, Fukui 917-0003, Japan.
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MeSH Terms
Amino Acid Sequence
Embryo, Nonmammalian
Gene Expression
Gene Expression Profiling
Molecular Sequence Data
Polymerase Chain Reaction
RNA, Messenger / metabolism
Sequence Homology, Amino Acid
Takifugu / embryology*,  metabolism*
Tissue Distribution*
Tissue Inhibitor of Metalloproteinase-3 / metabolism*
Tissue Inhibitor of Metalloproteinases / metabolism*
Reg. No./Substance:
0/RNA, Messenger; 0/Tissue Inhibitor of Metalloproteinase-3; 0/Tissue Inhibitor of Metalloproteinases; 0/tissue inhibitor of metalloproteinase-4

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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