Document Detail

Expression of four growth factors during fracture repair.
MedLine Citation:
PMID:  7514025     Owner:  NLM     Status:  MEDLINE    
Fracture repair offers an opportunity to study the physiology of bone formation at the fracture site. Isolation of growth factors from bone matrix has implicated growth factors as participants in bone physiology. We therefore examined the expression patterns of aFGF, IGF-I, PDGF, and TGF-beta during fracture repair. An animal model has been developed to study repair of tibial fractures. The model provides both reproducible and quantifiable results, allowing the fracture repair process to be divided into four stages (Bourque et al., Lab. Anim. Sci 42: 369-374, 1992). Fractured tibiae were examined immunohistochemically with polyclonal antibodies to four growth factors. PDGF was visualized in macrophages in close proximity to the periosteum during stage 1. aFGF was visualized in cells of the expanded cambial layer and was associated with a rapid increase in the population of fibroblast-like mesenchymal cells during stage 2. IGF-I was visualized in young chondroblasts at the edge of the cartilage mass replacing the fibrous callus during stage 3. TGF-beta was visualized in calcified matrix producing chondrocytes at the edge of ossification fronts penetrating the cartilage callus during stage 4. The immunohistochemical results suggest that these growth factors act as local simulators of the repair process.
W T Bourque; M Gross; B K Hall
Related Documents :
11764215 - Acetabular osteitis in ankylosing spondylitis: does fibrillin figure in its pathogenesis?
6522825 - Consequences of capital femoral dyschondroplasia in young adult and skeletally mature b...
7514025 - Expression of four growth factors during fracture repair.
8012265 - Calcified plate and flattening of the femoral condyle with spontaneous osteonecrosis.
1939305 - Salvage of ear cartilage in patients with acute full-thickness burns.
15034605 - Bone defects of the facial skeleton - replacement with biomaterials.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The International journal of developmental biology     Volume:  37     ISSN:  0214-6282     ISO Abbreviation:  Int. J. Dev. Biol.     Publication Date:  1993 Dec 
Date Detail:
Created Date:  1994-06-16     Completed Date:  1994-06-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8917470     Medline TA:  Int J Dev Biol     Country:  SPAIN    
Other Details:
Languages:  eng     Pagination:  573-9     Citation Subset:  IM    
Biology Department, Dalhousie University, Halifax, Nova Scotia, Canada.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cartilage / blood supply,  pathology,  physiopathology
Fibroblast Growth Factor 1 / analysis,  physiology
Fracture Healing / physiology*
Fractures, Bone / pathology,  physiopathology
Growth Substances / analysis,  physiology*
Immunoenzyme Techniques
Insulin-Like Growth Factor I / analysis,  physiology
Macrophages / physiology
Monocytes / physiology
Osteoblasts / physiology
Platelet-Derived Growth Factor / analysis,  physiology
Tibia / injuries
Transforming Growth Factor beta / analysis,  physiology
Reg. No./Substance:
0/Growth Substances; 0/Platelet-Derived Growth Factor; 0/Transforming Growth Factor beta; 104781-85-3/Fibroblast Growth Factor 1; 67763-96-6/Insulin-Like Growth Factor I

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Analysis of the endocytic-lysosomal system (vacuolar apparatus) in astrocytes during proliferation a...
Next Document:  Analyses of p53 antibodies in sera of patients with lung carcinoma define immunodominant regions in ...