| Expression of follicle-stimulating hormone receptor in tumor blood vessels. | |
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MedLine Citation:
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PMID: 20961245 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: In adult humans, the follicle-stimulating hormone (FSH) receptor is expressed only in the granulosa cells of the ovary and the Sertoli cells of the testis. It is minimally expressed by the endothelial cells of gonadal blood vessels. METHODS: We used immunohistochemical and immunoblotting techniques involving four separate FSH-receptor-specific monoclonal antibodies that recognize different FSH receptor epitopes and in situ hybridization to detect FSH receptor in tissue samples from patients with a wide range of tumors. Immunoelectron microscopy was used to detect FSH receptor in mouse tumors. RESULTS: In all 1336 patients examined, FSH receptor was expressed by endothelial cells in tumors of all grades, including early T1 tumors. The tumors were located in the prostate, breast, colon, pancreas, urinary bladder, kidney, lung, liver, stomach, testis, and ovary. In specimens obtained during surgery performed to remove tumors, the FSH receptor was not expressed in the normal tissues located more than 10 mm from the tumors. The tumor lymphatic vessels did not express FSH receptor. The endothelial cells that expressed FSH receptor were located at the periphery of the tumors in a layer that was approximately 10 mm thick; this layer extended both into and outside of the tumor. Immunoelectron microscopy in mice with xenograft tumors, after perfusion with anti–FSH-receptor antibodies coupled to colloidal gold, showed that the FSH receptor is exposed on the luminal endothelial surface and can bind and internalize circulating ligands. CONCLUSIONS: FSH receptor is selectively expressed on the surface of the blood vessels of a wide range of tumors. (Funded by INSERM.). |
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Authors:
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Aurelian Radu; Christophe Pichon; Philippe Camparo; Martine Antoine; Yves Allory; Anne Couvelard; Gaëlle Fromont; Mai Thu Vu Hai; Nicolae Ghinea |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The New England journal of medicine Volume: 363 ISSN: 1533-4406 ISO Abbreviation: N. Engl. J. Med. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-10-21 Completed Date: 2010-10-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0255562 Medline TA: N Engl J Med Country: United States |
Other Details:
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Languages: eng Pagination: 1621-30 Citation Subset: AIM; IM |
Affiliation:
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Mount Sinai School of Medicine, New York, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Monoclonal / analysis Blood Vessels / chemistry Disease Models, Animal Electrophoresis, Polyacrylamide Gel Endothelial Cells / chemistry* Humans Immunologic Techniques In Situ Hybridization Male Mice Microscopy, Immunoelectron Neoplasm Transplantation Neoplasms / blood supply*, chemistry* Neoplasms, Experimental / blood supply, chemistry Prostatic Neoplasms / blood supply*, chemistry Receptors, FSH / analysis*, immunology Transplantation, Heterologous |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Receptors, FSH |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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