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Expression of fibroblast growth factor by F9 teratocarcinoma cells as a function of differentiation.
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MedLine Citation:
PMID:  2544608     Owner:  NLM     Status:  MEDLINE    
F9 teratocarcinoma stem cells treated with retinoic acid (RA) and dibutyryl cAMP (but2 cAMP) differentiate into embryonic parietal endoderm. Using heparin-affinity chromatography, endothelial cell proliferation assays, immunoprecipitation, and Western analysis with antibodies specific for acidic and basic fibroblast growth factors (FGFs), we detected biologically active FGF in F9 cells only after differentiation. A bovine basic FGF cDNA probe hybridized to 2.2-kb mRNAs in both F9 stem and parietal endoderm cells and to a 3.8-kb mRNA in F9 stem cells. A genomic DNA probe for acidic FGF hybridized to a 5.8-6.0-kb mRNA in both F9 stem and parietal endoderm cells, and to a 6.0-6.3-kb mRNA only in parietal endoderm cells. Although these FGF mRNAs were present in the stem cells, we could find no evidence that F9 stem cells synthesized FGFs, whereas differentiated F9 cells synthesized both acidic and basic FGF-like proteins. We conclude that biologically active factors with properties characteristic of acidic and basic FGF are expressed by F9 parietal endoderm cells after differentiation. Differentiating embryonic parietal endoderm thus may serve as a source of FGF molecules in the developing blastocyst, where these factors appear to play a central role in subsequent embryogenesis.
S J Braunhut; L J Gudas; T Kurokawa; J Sasse; P A D'Amore
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of cell biology     Volume:  108     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1989 Jun 
Date Detail:
Created Date:  1989-08-04     Completed Date:  1989-08-04     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2467-76     Citation Subset:  IM    
Department of Ophthalmology, Children's Hospital, Boston, Massachusetts 02115.
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MeSH Terms
Blotting, Northern
Blotting, Western
Bucladesine / pharmacology
Cell Differentiation* / drug effects
DNA Probes
Embryonal Carcinoma Stem Cells
Fibroblast Growth Factors / physiology*
Heparin / metabolism
Neoplastic Stem Cells / cytology,  physiology
Teratoma / genetics,  pathology*
Tretinoin / pharmacology
Tumor Cells, Cultured
Grant Support
Reg. No./Substance:
0/DNA Probes; 302-79-4/Tretinoin; 362-74-3/Bucladesine; 62031-54-3/Fibroblast Growth Factors; 9005-49-6/Heparin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 6 Year: 1989
Volume: 108 Issue: 6
First Page: 2467 Last Page: 2476
ID: 2115611
Publisher Id: 89291983
PubMed Id: 2544608

Expression of fibroblast growth factor by F9 teratocarcinoma cells as a function of differentiation

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