| Expression of fibroblast growth factor by F9 teratocarcinoma cells as a function of differentiation. | |
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MedLine Citation:
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PMID: 2544608 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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F9 teratocarcinoma stem cells treated with retinoic acid (RA) and dibutyryl cAMP (but2 cAMP) differentiate into embryonic parietal endoderm. Using heparin-affinity chromatography, endothelial cell proliferation assays, immunoprecipitation, and Western analysis with antibodies specific for acidic and basic fibroblast growth factors (FGFs), we detected biologically active FGF in F9 cells only after differentiation. A bovine basic FGF cDNA probe hybridized to 2.2-kb mRNAs in both F9 stem and parietal endoderm cells and to a 3.8-kb mRNA in F9 stem cells. A genomic DNA probe for acidic FGF hybridized to a 5.8-6.0-kb mRNA in both F9 stem and parietal endoderm cells, and to a 6.0-6.3-kb mRNA only in parietal endoderm cells. Although these FGF mRNAs were present in the stem cells, we could find no evidence that F9 stem cells synthesized FGFs, whereas differentiated F9 cells synthesized both acidic and basic FGF-like proteins. We conclude that biologically active factors with properties characteristic of acidic and basic FGF are expressed by F9 parietal endoderm cells after differentiation. Differentiating embryonic parietal endoderm thus may serve as a source of FGF molecules in the developing blastocyst, where these factors appear to play a central role in subsequent embryogenesis. |
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Authors:
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S J Braunhut; L J Gudas; T Kurokawa; J Sasse; P A D'Amore |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of cell biology Volume: 108 ISSN: 0021-9525 ISO Abbreviation: J. Cell Biol. Publication Date: 1989 Jun |
Date Detail:
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Created Date: 1989-08-04 Completed Date: 1989-08-04 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0375356 Medline TA: J Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2467-76 Citation Subset: IM |
Affiliation:
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Department of Ophthalmology, Children's Hospital, Boston, Massachusetts 02115. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Northern Blotting, Western Bucladesine / pharmacology Cell Differentiation* / drug effects DNA Probes Embryonal Carcinoma Stem Cells Fibroblast Growth Factors / physiology* Heparin / metabolism Mice Neoplastic Stem Cells / cytology, physiology Teratoma / genetics, pathology* Tretinoin / pharmacology Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
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EY-05985/EY/NEI NIH HHS; EY-06726/EY/NEI NIH HHS; R0I HD-24319/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA Probes; 302-79-4/Tretinoin; 362-74-3/Bucladesine; 62031-54-3/Fibroblast Growth Factors; 9005-49-6/Heparin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Cell Biol ISSN: 0021-9525 ISSN: 1540-8140 Publisher: The Rockefeller University Press |
Article Information Download PDF  Print publication date: Day: 1 Month: 6 Year: 1989 Volume: 108 Issue: 6 First Page: 2467 Last Page: 2476 ID: 2115611 Publisher Id: 89291983 PubMed Id: 2544608 |
| Expression of fibroblast growth factor by F9 teratocarcinoma cells as a function of differentiation | |
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