| Expression of extracellular matrix genes in cultured hepatic oval cells: an origin of hepatic stellate cells through transforming growth factor beta? | |
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MedLine Citation:
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PMID: 19323784 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Hepatic oval cells, progenitor cells in the liver, can differentiate into hepatocytes and bile duct cells both in vitro and in vivo. Although hepatic stellate cells are another important cell component in the liver, less attention has been focused on the relationship between hepatic oval cells and hepatic stellate cells. METHODS: Hepatic oval cells were isolated from rats fed a choline-deficient diet supplemented with 0.1% ethionine for 6 weeks and characterized by electron microscopy, flow cytometry, reverse transcription polymerase chain reaction, Western blot and bi-direction differentiation. After treatment with transforming growth factor-beta1 (TGF-beta1), changes in cell viability, morphology, extracellular matrix (ECM) expression and immune phenotype were analysed in these cultured and adherent hepatic oval cells. RESULTS: The primary cultured hepatic oval cells were positive for the oval cell-specific markers OV-6, BD-1/BD-2 and M2PK as well as the hepatocyte markers albumin and alpha-foetoprotein. These hepatic oval cells differentiated bipotentially into hepatocytes or bile duct-like cells under appropriate conditions. It is noteworthy that these bipotential hepatic oval cells expressed ECM genes stably, including collagens, matrix metalloproteinases and tissue inhibitor of mellatoproteinase. Furthermore, except for growth inhibition and morphological changes in the hepatic oval cells after exposure to TGF-beta1, there was an increased expression of ECM genes, the onset expression of snail and loss expression of E-cadherin. During this process, TGF-beta1 treatment induced an upregulation of marker genes for hepatic stellate cells in hepatic oval cells, such as desmin and GFAP. CONCLUSION: Except for the expression of ECM, the cultured hepatic oval cells could induce an increased expression of hepatic stellate cell markers by TGF-beta1 through an epithelial-mesenchymal transition process, which might indicate the contribution of hepatic oval cells to liver fibrosis. |
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Authors:
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Ping Wang; Tianhui Liu; Min Cong; Xiaoning Wu; Yanfeng Bai; Chenghong Yin; Wei An; Baoen Wang; Jidong Jia; Hong You |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Liver international : official journal of the International Association for the Study of the Liver Volume: 29 ISSN: 1478-3231 ISO Abbreviation: Liver Int. Publication Date: 2009 Apr |
Date Detail:
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Created Date: 2009-03-27 Completed Date: 2009-06-25 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101160857 Medline TA: Liver Int Country: England |
Other Details:
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Languages: eng Pagination: 575-84 Citation Subset: IM |
Affiliation:
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Liver Research Center, Beijing Friendship Hospital, Capital Medical University, Beijing, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Albumins
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metabolism Animals Antimetabolites / administration & dosage, adverse effects Bile Ducts / drug effects, metabolism, pathology Biological Markers / metabolism Cadherins / metabolism Cell Differentiation / drug effects Cell Survival / drug effects Cells, Cultured Choline Deficiency / etiology, metabolism, pathology Desmin / genetics, metabolism Disease Models, Animal Ethionine / administration & dosage, adverse effects Extracellular Matrix Proteins / drug effects, genetics, metabolism*, pharmacology Gene Expression / drug effects* Glial Fibrillary Acidic Protein / genetics, metabolism Hepatocytes / drug effects, metabolism, pathology Liver / metabolism*, pathology Male RNA, Messenger / metabolism Rats Rats, Sprague-Dawley Stem Cells / drug effects, metabolism*, ultrastructure Transforming Growth Factor beta / metabolism*, pharmacology alpha-Fetoproteins / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Albumins; 0/Antimetabolites; 0/Biological Markers; 0/Cadherins; 0/Desmin; 0/Extracellular Matrix Proteins; 0/Glial Fibrillary Acidic Protein; 0/RNA, Messenger; 0/Transforming Growth Factor beta; 0/alpha-Fetoproteins; 13073-35-3/Ethionine; 148710-76-3/betaIG-H3 protein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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