Document Detail


Expression of estrogen receptor sub-types alpha and beta in acute and chronic cardiac allograft vasculopathy.
MedLine Citation:
PMID:  11744408     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The vasculoprotective effects of estrogen are well-established not only in women with age-related atherosclerosis, but also after experimental vascular injury and in chronic allograft vasculopathy. Evidence exists that the newly discovered estrogen receptor (ER) beta, rather than the classical ERalpha is related to the vasculoprotective effect. Here we investigate whether and to what extent the two ERs are expressed in cardiac allografts in the rat and human in native state and during acute and chronic rejection. METHODS: Rat cardiac allografts were performed from male DA (AG-B4, RT1(a)) to male WF (AG-B2, RT1(v)) strain and syngeneic transplants from DA to DA strain; human male-to-male heart allograft endomyocardial biopsies came from our biopsy files. RESULTS: Under in situ hybridization, ERbeta mRNA was prominently expressed in rat vessels and stroma, whereas ERalpha mRNA was present in low levels only. In immunohistochemistry, 2 ERbeta-specific antibodies stained rat and human vessels and stroma, whereas only a weak or no signal was obtained with 2 ERalpha-specific antibodies. Interestingly, the mRNA and protein expression levels in the rat carried only a weak correlation with the intensity of acute rejection, i.e., was not related to the intensity of inflammation. CONCLUSIONS: Our results demonstrate that ERbeta is the predominant ER in rat and human cardiac allografts, and suggest that, unless additional ERs are identified, the vasculoprotective effects of estrogen derivatives in cardiac allograft vasculopathy are mediated by ERbeta rather than by the classical ERalpha.
Authors:
H Savolainen; J Frösen; L Petrov; E Aavik; P Häyry
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation     Volume:  20     ISSN:  1053-2498     ISO Abbreviation:  J. Heart Lung Transplant.     Publication Date:  2001 Dec 
Date Detail:
Created Date:  2001-12-17     Completed Date:  2002-03-18     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9102703     Medline TA:  J Heart Lung Transplant     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1252-64     Citation Subset:  IM    
Affiliation:
Transplantation Laboratory, and the Rational Drug Design Programme, Biomedicum, University of Helsinki and Helsinki University Central Hospital, Helsinki, Finland. Hanna.Savolainen@Helsinki.Fi
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MeSH Terms
Descriptor/Qualifier:
Animals
Biopsy
Coronary Artery Disease / genetics*,  pathology
Coronary Vessels / pathology
Endocardium / pathology
Estrogen Receptor alpha
Estrogen Receptor beta
Graft Rejection / genetics*,  pathology
Heart Transplantation / pathology*
Humans
Male
Myocardium / pathology
Postoperative Complications / pathology*
RNA, Messenger / genetics*
Rats
Rats, Inbred Strains
Rats, Inbred WF
Receptors, Estrogen / genetics*
Transplantation, Homologous
Chemical
Reg. No./Substance:
0/Estrogen Receptor alpha; 0/Estrogen Receptor beta; 0/RNA, Messenger; 0/Receptors, Estrogen

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