| Expression of endothelin 1 in rat random-pattern skin flaps treated with topical nifedipine. | |
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MedLine Citation:
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PMID: 12533145 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To evaluate the relative tissue concentrations of the endogenous vasoactive peptide endothelin 1 (ET-1) in random-pattern skin flaps (RPSF) treated with either topical anti-ischemic drug therapy (nifedipine) or placebo. DESIGN: Prospective, randomized, placebo-controlled therapeutic trial. SUBJECTS: Adult male Sprague-Dawley rats. INTERVENTION: Experimental subjects underwent caudally based RPSFs using the modified McFarlane technique. Subjects received either topical anti-ischemic drug therapy (nifedipine; n = 6) or inert carrier ointment (placebo; n = 6). Treatment was initiated immediately following flap closure and continued every 6 hours for 5 days. At the end of the treatment period, the animals were killed and the concentration of ET-1 was determined using enzyme-linked immunosorbent assay. Representative tissues from nifedipine- and placebo-treated skin flaps were also analyzed for ET-1 using immunohistochemical stains. RESULTS: The ET-1 levels in the distal (necrotic) flap segments were increased by 4.53 pg/mL over baseline (nonnecrotic) flaps in the placebo-treated animals and decreased by 4.70 pg/mL below baseline in the nifedipine-treated group (P =.03). CONCLUSIONS: The correlation between tissue levels of ET-1 and the severity of tissue necrosis suggests that ET-1 may play a pivotal role in ischemic injury of RPSFs. Moreover, treatment with topical nifedipine may antagonize the vasoconstrictive effects of ET-1. Although immunohistochemical analysis revealed ET-1 staining within the flap microvasculature, no quantitative differences were detected between the nifedipine- and placebo-treated flaps. Further studies are needed to define the role of ET-1 in RPSF necrosis. |
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Authors:
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Steven Ross Mobley; Marilyn K Glassberg; Anne Luebke; Chad A Perlyn; Richard E Davis |
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Publication Detail:
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Type: Evaluation Studies; Journal Article |
Journal Detail:
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Title: Archives of facial plastic surgery Volume: 5 ISSN: 1521-2491 ISO Abbreviation: Arch Facial Plast Surg Publication Date: 2003 Jan-Feb |
Date Detail:
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Created Date: 2003-01-20 Completed Date: 2003-06-06 Revised Date: 2012-09-24 |
Medline Journal Info:
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Nlm Unique ID: 100883500 Medline TA: Arch Facial Plast Surg Country: United States |
Other Details:
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Languages: eng Pagination: 78-82 Citation Subset: IM |
Affiliation:
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Divisions of Facial Plastic and Reconstructive Surgery, Department of Otolaryngology, University of Miami School of Medicine, Miami, FL, USA |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Administration, Topical Animals Endothelin-1 / biosynthesis* Male Models, Animal Necrosis Nifedipine / administration & dosage, pharmacology* Prospective Studies Random Allocation Rats Rats, Sprague-Dawley Skin / blood supply, drug effects*, metabolism*, pathology Surgical Flaps / blood supply*, physiology Vasodilator Agents / administration & dosage, pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Endothelin-1; 0/Vasodilator Agents; 21829-25-4/Nifedipine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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