Document Detail


Expression of the eicosapentaenoic acid synthesis gene cluster from Shewanella sp. in a transgenic marine cyanobacterium, Synechococcus sp.
MedLine Citation:
PMID:  9274025     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The eicosapentaenoic acid (EPA) synthesis gene cluster isolated from a marine bacterium, Shewanella putrefaciens strain SCRC-2738, was cloned and expressed in the marine cyanobacterium Synechococcus sp. A broad-host-range cosmid vector, pJRD215 (10.2 kb, Smr Kmr), was used to clone a 38 kb insert, pEPA, containing the EPA synthesis gene cluster, creating plasmid pJRDEPA (approx. 48 kb). This plasmid was transferred to the cyanobacterial host at a frequency of 2.2 x 10(-7). Cyanobacterial transconjugants grown at 29 degrees C produced 0.12 mg EPA (g dry weight)-1, whereas those grown at 23 degrees C produced 0.56 mg EPA (g dry weight)-1. The yield was further improved to 0.64 mg (g dry weight)-1 by incubation for 1 d at 17 degrees C. This is believed to be the first successful cloning and expression of such a large heterologous gene cluster in a marine cyanobacterium.
Authors:
H Takeyama; D Takeda; K Yazawa; A Yamada; T Matsunaga
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Microbiology (Reading, England)     Volume:  143 ( Pt 8)     ISSN:  1350-0872     ISO Abbreviation:  Microbiology (Reading, Engl.)     Publication Date:  1997 Aug 
Date Detail:
Created Date:  1997-10-06     Completed Date:  1997-10-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9430468     Medline TA:  Microbiology     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  2725-31     Citation Subset:  IM    
Affiliation:
Tokyo University of Agriculture and Technology, Department of Biotechnology, Japan.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/U73935
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MeSH Terms
Descriptor/Qualifier:
Conjugation, Genetic
Cyanobacteria / genetics,  metabolism*
Dietary Fats
Eicosapentaenoic Acid / biosynthesis*,  chemistry
Escherichia coli / genetics
Gas Chromatography-Mass Spectrometry
Gene Expression
Genes, Bacterial*
Genetic Engineering
Gram-Negative Facultatively Anaerobic Rods / genetics*
Marine Biology
Molecular Sequence Data
Multigene Family*
Chemical
Reg. No./Substance:
0/Dietary Fats; 1553-41-9/Eicosapentaenoic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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