Document Detail


Expression dynamics of the Medicago truncatula transcriptome during the symbiotic interaction with Sinorhizobium meliloti: which role for nitric oxide?
MedLine Citation:
PMID:  23136381     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Medicago truncatula is one of the most studied model plants. Nevertheless, the genome of this legume remains incompletely determined. We used RNA-Seq to characterize the transcriptome during the early organogenesis of the nodule and during its functioning. We detected 37,333 expressed transcription units; to our knowledge, 1,670 had never been described before and were functionally annotated. We identified 7,595 new transcribed regions, mostly corresponding to 5' and 3' untranslated region extensions and new exons associated with 5,264 previously annotated genes. We also inferred 23,165 putative transcript isoforms from 6,587 genes and measured the abundance of transcripts for each isoform, which suggests an important role for alternative splicing in the generation of proteome diversity in M. truncatula. Finally, we carried out a differential expression analysis, which provided a comprehensive view of transcriptional reprogramming during nodulation. In particular, depletion of nitric oxide in roots inoculated with Sinorhizobium meliloti greatly increased our understanding of the role of this reactive species in the optimal establishment of the symbiotic interaction, revealing differential patterns of expression for 2,030 genes and pointing to the inhibition of the expression of defense genes.
Authors:
Alexandre Boscari; Jennifer Del Giudice; Alberto Ferrarini; Luca Venturini; Anne-Lise Zaffini; Massimo Delledonne; Alain Puppo
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-11-07
Journal Detail:
Title:  Plant physiology     Volume:  161     ISSN:  1532-2548     ISO Abbreviation:  Plant Physiol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2012-12-31     Completed Date:  2013-06-18     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  425-39     Citation Subset:  IM    
Affiliation:
Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1355, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 7254, and Université de Nice-Sophia Antipolis, Unité Mixte de Recherche Institut Sophia Agrobiotech, 06903 Sophia Antipolis, France.
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MeSH Terms
Descriptor/Qualifier:
3' Untranslated Regions
5' Untranslated Regions
Alternative Splicing
Exons
Gene Expression Regulation, Plant
Genes, Plant
High-Throughput Nucleotide Sequencing
Introns
Medicago truncatula / genetics,  metabolism,  microbiology*
Molecular Sequence Annotation
Nitric Oxide / metabolism*
Plant Root Nodulation
Plant Roots / metabolism,  microbiology
RNA, Plant / genetics
Sinorhizobium meliloti / growth & development*
Symbiosis*
Transcription Factors / genetics,  metabolism
Transcription, Genetic
Transcriptome*
Chemical
Reg. No./Substance:
0/3' Untranslated Regions; 0/5' Untranslated Regions; 0/RNA, Plant; 0/Transcription Factors; 10102-43-9/Nitric Oxide
Comments/Corrections

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