Document Detail


Expression of double Vitreoscilla hemoglobin enhances growth and alters ribosome and tRNA levels in Escherichia coli.
MedLine Citation:
PMID:  12052087     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In several organisms, expression of a gene encoding dimeric hemoglobin (VHb) from the obligate aerobic bacterium Vitreoscilla stercoraria has been shown to increase microaerobic cell growth and enhance oxygen-dependent cell metabolism. In an attempt to further improve these effects of VHb, a gene encoding two vhb genes connected by a short linker of six base pairs was constructed and expressed in Escherichia coli(double VHb). Escherichia coli cells expressing double VHb reached a cell density 19% higher than that of cells expressing native VHb. The protein production per cell remained constant since the increase in cell growth was accompanied by an increase in protein content by 16%. Investigation of ribosome and tRNA content revealed that cells expressing double VHb reached their maximal capacity of protein synthesis later during cultivation than cells expressing native VHb, and furthermore they reached considerably higher levels of ribosome and tRNA compared to that of the VHb-expressing cells.
Authors:
Viktoria Roos; Charlotte I J Andersson; Cecilia Arfvidsson; Karl-Gustav Wahlund; Leif Bülow
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology progress     Volume:  18     ISSN:  8756-7938     ISO Abbreviation:  Biotechnol. Prog.     Publication Date:    2002 May-Jun
Date Detail:
Created Date:  2002-06-07     Completed Date:  2002-12-30     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8506292     Medline TA:  Biotechnol Prog     Country:  United States    
Other Details:
Languages:  eng     Pagination:  652-6     Citation Subset:  IM    
Affiliation:
Department of Pure and Applied Biochemistry, Center for Chemistry and Chemical Engineering, Lund University, Lund, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / chemistry,  genetics*,  metabolism
Base Sequence
Carbon Monoxide / metabolism
DNA Primers
Electrophoresis, Polyacrylamide Gel
Escherichia coli / genetics*,  growth & development
Hemoglobins / chemistry,  genetics*,  metabolism
Models, Molecular
Plasmids
RNA, Transfer / genetics,  metabolism*
Ribosomes / metabolism*
Truncated Hemoglobins
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA Primers; 0/Hemoglobins; 0/Truncated Hemoglobins; 104781-86-4/hemoglobin protein, Vitreoscilla; 630-08-0/Carbon Monoxide; 9014-25-9/RNA, Transfer

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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