Document Detail


Expression of a dominant negative form of Daxx in vivo rescues motoneurons from Fas (CD95)-induced cell death.
MedLine Citation:
PMID:  15459896     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fas-induced death of motoneurons in vitro has been shown to involve two signaling cascades that act together to execute the death program: a Fas-Daxx-ASK-1-p38 kinase-nNOS branch, which controls transcriptional and post-translational events, and the second classical Fas-FADD-caspase-8 branch. To analyze the role of Daxx in the developmental motoneuron cell death, we studied Fas-dependent cell death in motoneurons from transgenic mice that overexpress a dominant-negative form of Daxx. Motoneurons purified from these transgenic mice are resistant to Fas-induced death. This protective effect is specific to Fas because ultraviolet irradiation-triggered death is not affected by the transgene. The Daxx and the FADD pathways work in parallel because only Daxx, but not FADD, is involved in the transcriptional control of neuronal nitric oxide synthase and nitric oxide production. Nevertheless, we do not observe involvement of Daxx in developmental motoneuronal cell death, as the pattern of naturally occurring programmed cell death in vivo is normal in transgenic mice overexpressing the dominant negative form of Daxx, suggesting that Daxx-independent pathways are used during development.
Authors:
Cedric Raoul; Catherine Barthelemy; Arnaud Couzinet; David Hancock; Brigitte Pettmann; Anne-Odile Hueber
Related Documents :
12543166 - Sensitization of head and neck squamous cell carcinoma cells to fas-mediated apoptosis ...
14694156 - The death domain of kidney ankyrin interacts with fas and promotes fas-mediated cell de...
18078776 - Fas/fas-l expression, apoptosis and low proliferative response are associated with hear...
9182976 - Sensitization of cancer cells treated with cytotoxic drugs to fas-mediated cytotoxicity.
23353896 - Molecular mechanism of anti-inflammatory activity of pluchea indica leaves in macrophag...
22815836 - Cord blood leptin levels of healthy neonates are associated with ifn-γ production by c...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neurobiology     Volume:  62     ISSN:  0022-3034     ISO Abbreviation:  J. Neurobiol.     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2004-12-14     Completed Date:  2005-04-11     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0213640     Medline TA:  J Neurobiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  178-88     Citation Subset:  IM    
Copyright Information:
2004 Wiley Periodicals, Inc
Affiliation:
INSERM U. 382, Developmental Biology Institute of Marseille (CNRS-INSERM-Univ. Méditerranée-AP Marseille), Campus de Luminy-Case 907, 13288 Marseille, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adaptor Proteins, Signal Transducing / metabolism
Animals
Antigens, CD95 / physiology*
Apoptosis* / radiation effects
Carrier Proteins / genetics,  metabolism*
Cell Count / methods
Embryo, Mammalian
Fas Ligand Protein
Fluorescent Antibody Technique / methods
Genes, Dominant
Green Fluorescent Proteins / metabolism
In Situ Nick-End Labeling / methods
Indoles / diagnostic use
Intracellular Signaling Peptides and Proteins / genetics,  metabolism*
JNK Mitogen-Activated Protein Kinases / metabolism
Membrane Glycoproteins
Mice
Mice, Inbred C57BL
Mice, Transgenic
Motor Neurons / cytology*
Nerve Tissue Proteins / metabolism
Nitric Oxide Synthase / metabolism
Nitric Oxide Synthase Type I
Nuclear Proteins / genetics,  metabolism*
Peptides / metabolism
RNA, Messenger / biosynthesis
Reverse Transcriptase Polymerase Chain Reaction / methods
Spinal Cord / cytology*
Time Factors
Chemical
Reg. No./Substance:
0/Adaptor Proteins, Signal Transducing; 0/Antigens, CD95; 0/Carrier Proteins; 0/Daxx protein, mouse; 0/Fas Ligand Protein; 0/Fasl protein, mouse; 0/Indoles; 0/Intracellular Signaling Peptides and Proteins; 0/Membrane Glycoproteins; 0/Nerve Tissue Proteins; 0/Nuclear Proteins; 0/Peptides; 0/RNA, Messenger; 147336-22-9/Green Fluorescent Proteins; 47165-04-8/DAPI; 98849-88-8/FLAG peptide; EC 1.14.13.39/Nitric Oxide Synthase; EC 1.14.13.39/Nitric Oxide Synthase Type I; EC 1.14.13.39/Nos1 protein, mouse; EC 2.7.11.24/JNK Mitogen-Activated Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Neural song preference during vocal learning in the zebra finch depends on age and state.
Next Document:  Dose and age-dependent axonal responses of embryonic trigeminal neurons to localized NGF via p75NTR ...