| Expression and distribution of cytokeratin 8/18 intermediate filaments in bovine antral follicles and corpus luteum: an intrinsic mechanism of resistance to apoptosis? | |
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MedLine Citation:
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PMID: 20503177 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Apoptosis is a mechanism of cell elimination during follicular atresia and luteal regression. Recent evidence suggests sensitivity to apoptosis in some cell types is partly dependent upon cytokeratin-containing intermediate filaments. Specifically, cytokeratin 8/18 (CK8/18) filaments are thought to impart resistance to apoptosis. Here, cytokeratin filament expression within bovine ovarian follicles and corpora lutea (CL) was characterized and the potential relationship between cell-specific CK8/18 expression and apoptosis explored. Immunoprecipitation and western blot analysis confirmed CK8 associates with CK18 to form CK8/18 heterodimeric filaments within bovine ovarian cells. Immunostaining revealed populations of CK18-positive (CK18+) cells in healthy growing follicles that increased in postovulatory follicles. Atretic follicles at all stages of atresia also contained some CK18+ cells. However, no CK18+ cells were detected in primordial or primary follicles. In CL, developing CL contained a higher proportion of CK18+ cells (approximately 35%, range 30-70%) than mature CL (approximately 16%) and regressing CL (approximately 5%; P<0.05, n = 3-5 CL/stage), suggesting CK8/18 filament expression diminishes over time, as luteal cells become more susceptible to apoptosis. Dual-fluorescence labeling for CK18 and a cell death marker (TUNEL labeling) confirmed this view, demonstrating less death of CK18+ than CK18- luteal cells throughout the estrous cycle (P<0.05). The results indicate differential expression of CK8/18 filaments occurs in cells of bovine ovarian follicles and CL throughout the estrous cycle. The prevalence and cell-specific pattern of cytokeratin expression in these structures is consistent with the concept these filaments might impart resistance to apoptosis in ovarian cells as is seen in other cell types. |
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Authors:
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David H Townson; Amanda N Putnam; Brian T Sullivan; Lankai Guo; Helen F Irving-Rodgers |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Histology and histopathology Volume: 25 ISSN: 1699-5848 ISO Abbreviation: Histol. Histopathol. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-05-26 Completed Date: 2010-09-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8609357 Medline TA: Histol Histopathol Country: Spain |
Other Details:
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Languages: eng Pagination: 889-900 Citation Subset: IM |
Affiliation:
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University of New Hampshire, Department of Molecular, Cellular and Biomedical Sciences, Durham, NH 03824, USA. dave.townson@unh.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis* Cattle Corpus Luteum / metabolism* Estrous Cycle Female Follicular Atresia / physiology In Situ Nick-End Labeling Keratin-18 / analysis, metabolism Keratins / analysis, metabolism Luteal Cells Ovarian Follicle* / cytology, metabolism, physiology Ovary / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Keratin-18; 68238-35-7/Keratins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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