Document Detail


Expression and distribution of cytokeratin 8/18 intermediate filaments in bovine antral follicles and corpus luteum: an intrinsic mechanism of resistance to apoptosis?
MedLine Citation:
PMID:  20503177     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Apoptosis is a mechanism of cell elimination during follicular atresia and luteal regression. Recent evidence suggests sensitivity to apoptosis in some cell types is partly dependent upon cytokeratin-containing intermediate filaments. Specifically, cytokeratin 8/18 (CK8/18) filaments are thought to impart resistance to apoptosis. Here, cytokeratin filament expression within bovine ovarian follicles and corpora lutea (CL) was characterized and the potential relationship between cell-specific CK8/18 expression and apoptosis explored. Immunoprecipitation and western blot analysis confirmed CK8 associates with CK18 to form CK8/18 heterodimeric filaments within bovine ovarian cells. Immunostaining revealed populations of CK18-positive (CK18+) cells in healthy growing follicles that increased in postovulatory follicles. Atretic follicles at all stages of atresia also contained some CK18+ cells. However, no CK18+ cells were detected in primordial or primary follicles. In CL, developing CL contained a higher proportion of CK18+ cells (approximately 35%, range 30-70%) than mature CL (approximately 16%) and regressing CL (approximately 5%; P<0.05, n = 3-5 CL/stage), suggesting CK8/18 filament expression diminishes over time, as luteal cells become more susceptible to apoptosis. Dual-fluorescence labeling for CK18 and a cell death marker (TUNEL labeling) confirmed this view, demonstrating less death of CK18+ than CK18- luteal cells throughout the estrous cycle (P<0.05). The results indicate differential expression of CK8/18 filaments occurs in cells of bovine ovarian follicles and CL throughout the estrous cycle. The prevalence and cell-specific pattern of cytokeratin expression in these structures is consistent with the concept these filaments might impart resistance to apoptosis in ovarian cells as is seen in other cell types.
Authors:
David H Townson; Amanda N Putnam; Brian T Sullivan; Lankai Guo; Helen F Irving-Rodgers
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Histology and histopathology     Volume:  25     ISSN:  1699-5848     ISO Abbreviation:  Histol. Histopathol.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-05-26     Completed Date:  2010-09-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8609357     Medline TA:  Histol Histopathol     Country:  Spain    
Other Details:
Languages:  eng     Pagination:  889-900     Citation Subset:  IM    
Affiliation:
University of New Hampshire, Department of Molecular, Cellular and Biomedical Sciences, Durham, NH 03824, USA. dave.townson@unh.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis*
Cattle
Corpus Luteum / metabolism*
Estrous Cycle
Female
Follicular Atresia / physiology
In Situ Nick-End Labeling
Keratin-18 / analysis,  metabolism
Keratins / analysis,  metabolism
Luteal Cells
Ovarian Follicle* / cytology,  metabolism,  physiology
Ovary / metabolism
Chemical
Reg. No./Substance:
0/Keratin-18; 68238-35-7/Keratins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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