Document Detail

Expression of α-defensin 1-3 in T cells from severe cutaneous drug-induced hypersensitivity reactions.
MedLine Citation:
PMID:  20880148     Owner:  NLM     Status:  In-Data-Review    
To cite this article: Morel E, Álvarez L, Cabañas R, Fiandor A, Díaz R, Escamochero S, Prior N, Blanca M, Bellón T. Expression of α-defensin 1-3 in T cells from severe cutaneous drug-induced hypersensitivity reactions. Allergy 2011; 66: 360-367. ABSTRACT: Background:  Cytotoxic T cells seem to be the main effector cells in Stevens-Johnson syndrome/toxic epidermal necrolysis (SJS/TEN). However, recent data support a role of the innate immune system in the etiopathology of drug-induced cutaneous reactions. In this study, we analyzed the expression of α-defensins 1-3 in mononuclear cells from patients with SJS/TEN, drug-induced maculopapular exanthema (MPE), and healthy donors. Methods:  DEFA1A3 gene expression was analyzed by quantitative and end-point RT-PCR. Intracellular flow cytometry, immunofluorescence and immunohistochemistry were carried out to verify α-defensin 1-3 protein expression in mononuclear cells from peripheral blood and skin infiltrates. α-Defensin 1-3 concentration was evaluated in plasma and blister fluid samples by ELISA. Results:  We herein describe DEFA1A3 gene expression in peripheral blood mononuclear cells (PBMCs) from patients with drug-induced cutaneous diseases. Gene expression analysis unveiled transcription in CD4 and CD8 peripheral blood T cells. Protein expression was confirmed by intracellular flow cytometry in mononuclear cells from the patients, including monocytes, NK cells, and T cells from peripheral blood and blister fluid. Further analysis of protein content by flow cytometry revealed higher protein levels in CD56(+) CD3(+) lymphocytes from patients with SJS/TEN when compared to MPE and healthy donors. Immunohistological analysis was used to confirm expression in dermal infiltrates. α-Defensin levels were estimated by ELISA to be 3- to 175-fold higher in blister fluid when compared to simultaneously drawn plasma samples. Conclusion:  Upregulation of innate immune molecules such as α-defensins 1-3 in T cells from patients with SJS/TEN may be involved in the etiopathology of these life-threatening diseases induced by medications.
E Morel; L Alvarez; R Cabañas; A Fiandor; R Díaz; S Escamochero; N Prior; M Blanca; T Bellón
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Publication Detail:
Type:  Journal Article     Date:  2010-09-29
Journal Detail:
Title:  Allergy     Volume:  66     ISSN:  1398-9995     ISO Abbreviation:  Allergy     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-02-02     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7804028     Medline TA:  Allergy     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  360-7     Citation Subset:  IM    
Copyright Information:
© 2010 John Wiley & Sons A/S.
Research Unit, Hospital Universitario La Paz-IdiPAZ, Madrid Allergy Department, Hospital La Paz, Madrid Dermatology Department, Hospital Infanta Sofía, Madrid Allergy Department, Hospital Carlos Haya, Málaga, Spain.
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