| Expression of cytokeratin 18 during pre- and post-natal porcine lung development. | |
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MedLine Citation:
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PMID: 12484419 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The expression pattern of the intermediate filament protein cytokeratin 18 (CK 18) is described during pre- and post-natal development of the porcine lung using a monoclonal antibody against human CK 18. Lungs from 16 foetuses in pseudoglandular, canalicular, saccular and alveolar stages of lung development and lungs from 12 pigs ranging in age from birth to 49 days after birth were studied by immunohistochemistry. In the early pseudoglandular stage of development (day 70 of gestation) all the columnar epithelial cells lining the tubular endbuds strongly expressed CK 18 predominantly in the apical cell compartment. A modest staining was found in the more cuboidal cells of the canalicular stage (day 80 of gestation) where the labelling occurred as a distinct positive rim at the apical cell membrane in most of the cells lining the canaliculi. In 96- and 100-day-old foetuses, parts of the gas exchanging area were formed as terminal sacs by extreme attenuation of the epithelium. In this stage, CK 18 was clearly detectable in the flat type I as well as in the cuboidal type II alveolar epithelial cells. A marked change of the CK 18 expression pattern occurred during formation of the alveoli by septal outgrowth and maturation of the epithelium in 105- and 111-day-old foetuses. Differentiated type I cells no longer expressed CK 18, whereas type II cells were still labelled. Moreover, a specific change in the subcellular distribution pattern from the luminal periphery in immature porcine type II cells to a cytoplasmic localization in differentiated type II cells could be observed. Our investigation additionally demonstrated that the epithelium of bronchi, bronchioli and terminal bronchioli expressed CK 18 in all pre- and post-natal developmental stages. From the 96 days of gestation onwards the epithelial cells of developing bronchial glands were also labelled. Our results clearly show that during porcine lung development profound changes in the cellular expression pattern of CK 18 occur and that CK 18 can be regarded as a selective marker for differentiated porcine alveolar type II cells from the 105th day of gestation onwards. We also assume that the intermediate filament CK 18 could be of significance in the maturation process of the type II alveolar cells. |
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Authors:
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H Schlichenmaier; M Steffl; F Sinowatz; W M Amselgruber |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Anatomia, histologia, embryologia Volume: 31 ISSN: 0340-2096 ISO Abbreviation: Anat Histol Embryol Publication Date: 2002 Oct |
Date Detail:
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Created Date: 2002-12-17 Completed Date: 2003-03-04 Revised Date: 2009-11-03 |
Medline Journal Info:
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Nlm Unique ID: 7704218 Medline TA: Anat Histol Embryol Country: Germany |
Other Details:
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Languages: eng Pagination: 273-7 Citation Subset: IM |
Affiliation:
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Institute of Anatomy and Physiology, University of Hohenheim, Stuttgart, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Age Factors Animals Cell Differentiation Epithelial Cells / cytology, metabolism Immunohistochemistry / veterinary Keratins / analysis, biosynthesis*, physiology Lung / embryology*, growth & development, metabolism* Swine / embryology*, growth & development, metabolism |
| Chemical | |
Reg. No./Substance:
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68238-35-7/Keratins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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