Document Detail

Expression of cystatin C prevents oxidative stress-induced death in PC12 cells.
MedLine Citation:
PMID:  16140167     Owner:  NLM     Status:  MEDLINE    
Cystatin C, an inhibitor of cysteine proteinases, is suggested to be involved in oxidative stress-induced apoptosis of cultured CNS neurons and various neuronal diseases in vivo; however, little is known about its mechanism of action. To address the role cystatin C plays in oxidative stress-induced neuronal cell death, we established PC12 cell lines that stably expressed rat cystatin C. These cystatin C-expressing PC12 cells showed remarkable resistance to high (50%) oxygen atmosphere. This resistance correlate with expression levels of cystatin C, demonstrating that cystatin C has a protective effect on high oxygen-induced cell death. In contrast, in a normal (20%) oxygen atmosphere neither control nor cystatin C-expressing PC12 cells showed a significant change in the number of living cells, indicating that cystatin C does not play an important role in the regulation of cellular proliferation. Furthermore, the cystatin C-expressing cell line also resisted other oxidative stresses, including glutamate- and 13-L-hydroperoxylinoleic acid (LOOH)-induced cell death. These results demonstrate that cystatin C has protective effects against various oxidative stresses that induce cell death.
Keiji Nishiyama; Akio Konishi; Chika Nishio; Kiyomi Araki-Yoshida; Hiroshi Hatanaka; Masami Kojima; Yoshihiro Ohmiya; Masashi Yamada; Hisatsugu Koshimizu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Brain research bulletin     Volume:  67     ISSN:  0361-9230     ISO Abbreviation:  Brain Res. Bull.     Publication Date:  2005 Sep 
Date Detail:
Created Date:  2005-09-05     Completed Date:  2005-12-13     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7605818     Medline TA:  Brain Res Bull     Country:  United States    
Other Details:
Languages:  eng     Pagination:  94-9     Citation Subset:  IM    
Division of Protein Biosynthesis, Institute for Protein Research, Osaka University, Suita, Japan.
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MeSH Terms
Apoptosis / drug effects,  physiology*
Brain / metabolism,  physiopathology
Cystatin C
Cystatins / genetics,  metabolism*,  pharmacology
Cysteine Endopeptidases / metabolism
Cytoprotection / drug effects,  physiology
Drug Resistance / physiology
Glutamic Acid / pharmacology
Neurodegenerative Diseases / metabolism,  physiopathology
Neurons / drug effects,  metabolism*
Neuroprotective Agents / metabolism*,  pharmacology
Oxidative Stress / drug effects,  physiology*
Oxygen / adverse effects
PC12 Cells
Reg. No./Substance:
0/Cst3 protein, rat; 0/Cystatin C; 0/Cystatins; 0/Neuroprotective Agents; 56-86-0/Glutamic Acid; 7782-44-7/Oxygen; EC 3.4.22.-/Cysteine Endopeptidases

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