Document Detail


Expression of cyclooxygenases in ductus arteriosus of fetal and newborn pigs.
MedLine Citation:
PMID:  9855607     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: We studied the ontogeny of the expression of cyclooxygenase-1 and cyclooxygenase-2 in the ductus arteriosus and evaluated their functional significance. STUDY DESIGN: The expression of cyclooxygenase-1 and cyclooxygenase-2 was studied in ductus arteriosus of fetal (at approximately 75% gestation) and term newborn pig. Effects of selective inhibitors of cyclooxygenase-1 and cyclooxygenase-2 on ductal patency were evaluated by Doppler ultrasonography. RESULTS: Ductus arteriosus of the fetus expressed virtually only cyclooxygenase-1 immunoreactive protein and activity. In contrast, the ductus of the term newborn pig (<45 minutes old) contained proteins of both cyclooxygenase-1 and cyclooxygenase-2, but the latter contributed to >90% of prostaglandin E2 formation. The selective cyclooxygenase-2 inhibitor DuP697 reduced prostaglandin E2 levels in the ductus arteriosus, albeit not in plasma, but did not affect ductal patency in the newborn pig (<1(1/2) hours old); in contrast, the cyclooxygenase-1 inhibitor valeryl salicylate, like indomethacin, markedly reduced levels of prostaglandin E2 in the plasma and ductus arteriosus and caused significant constriction of the ductus arteriosus. CONCLUSION: The ductus arteriosus of the term newborn pig, in contrast to that of the fetus, expresses cyclooxygenase-2, but circulating prostaglandins, arising mostly from cyclooxygenase-1, seem to exert the major control on ductal patency in vivo. Our data suggest that cyclooxygenase-2 inhibitors might be better alternatives for the fetus than nonselective cyclooxygenase blockers if indicated for maternal conditions such as inflammation or for tocolysis.
Authors:
A M Guerguerian; P Hardy; M Bhattacharya; P Olley; R I Clyman; J C Fouron; S Chemtob
Related Documents :
900167 - Prolonged rupture of fetal membranes and decreased frequency of respiratory distress sy...
7377887 - Management of patent ductus arteriosus in preterm babies.
3505267 - Chest physiotherapy in preterm infants with rds in the first 24 hours of life.
2498657 - A randomized, controlled trial of very early prophylactic ligation of the ductus arteri...
19301247 - Treatment of an infant with severe acute refractory immune thrombocytopenic purpura usi...
23586497 - Accuracy of amplitude-integrated electroencephalography in the prediction of neurodevel...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of obstetrics and gynecology     Volume:  179     ISSN:  0002-9378     ISO Abbreviation:  Am. J. Obstet. Gynecol.     Publication Date:  1998 Dec 
Date Detail:
Created Date:  1999-01-15     Completed Date:  1999-01-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370476     Medline TA:  Am J Obstet Gynecol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1618-26     Citation Subset:  AIM; IM    
Affiliation:
Departments of Pediatrics, Ophthalmology, and Pharmacology, University of Montreal, Canada.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn / metabolism*
Cyclooxygenase 1
Cyclooxygenase 2
Dinoprostone / biosynthesis
Ductus Arteriosus / drug effects,  enzymology*,  metabolism
Enzyme Inhibitors / pharmacology
Fetus / blood supply,  enzymology*
Isoenzymes / metabolism*
Prostaglandin-Endoperoxide Synthases / metabolism*
Swine
Chemical
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Isoenzymes; 363-24-6/Dinoprostone; EC 1.14.99.1/Cyclooxygenase 1; EC 1.14.99.1/Cyclooxygenase 2; EC 1.14.99.1/Prostaglandin-Endoperoxide Synthases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Abnormal ductus venosus blood flow in trisomy 21 fetuses during early pregnancy.
Next Document:  New Down syndrome screening algorithm: ultrasonographic biometry and multiple serum markers combined...