Document Detail

Expression of cyclo-oxygenase-2 in naturally occurring squamous cell carcinomas in dogs.
MedLine Citation:
PMID:  11410611     Owner:  NLM     Status:  MEDLINE    
Squamous cell carcinoma is one of the most common cancers in humans and is also a frequently diagnosed neoplasm in dogs. Induction of cyclo-oxygenase-2 (COX-2), a key rate-limiting enzyme in prostaglandin biosynthesis, has been implicated in the oncogenesis of various cancers in humans, including squamous cell carcinomas. However, expression of COX-2 has not been reported in spontaneous squamous cell carcinomas of non-human species. Canine squamous cell carcinomas share several similarities with the human disease. Therefore, the objective of this study was to determine whether COX isoenzymes were expressed in naturally occurring cases of squamous cell carcinomas in dogs. Canine normal skin (n=4) and squamous cell carcinomas (n=40) were studied by immunohistochemistry and immunoblotting analysis using polyclonal antibodies selective for COX-1 or COX-2. COX-2 was strongly expressed by neoplastic keratinocytes in all cases of squamous cell carcinomas, whereas no COX-2 was detected in normal skin and in the non-neoplastic skin and oral mucosa included in the tumor tissue samples (p<0.01). Immunoblotting analysis confirmed the restricted expression of COX-2 (72,000--74,000 molecular weight doublet) in squamous cell carcinomas only. In contrast, faint COX-1 staining was found in normal skin and in squamous cell carcinomas. This study demonstrates for the first time that COX-2 is induced in canine squamous cell carcinomas, and provides a new model to investigate the role and regulation of COX-2 gene expression in naturally occurring squamous cell carcinomas. (J Histochem Cytochem 49:867-875, 2001)
E M Pestili de Almeida; C Piché; J Sirois; M Doré
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  49     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  2001 Jul 
Date Detail:
Created Date:  2001-06-18     Completed Date:  2001-07-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  867-75     Citation Subset:  IM    
Département de Pathologie et Microbiologie, Faculté de Médecine Vétérinaire, Université de Montréal, St-Hyacinthe, Québec, Canada.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cyclooxygenase 1
Cyclooxygenase 2
Dog Diseases / enzymology*
Enzyme Induction
Isoenzymes / biosynthesis,  metabolism*
Neoplasms, Squamous Cell / enzymology*
Organ Specificity
Prostaglandin-Endoperoxide Synthases / biosynthesis,  metabolism*
Reg. No./Substance:
0/Isoenzymes; EC 1; EC 2; EC Synthases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Variations in dystrophin complex in red and white caudal muscles from Torpedo marmorata.
Next Document:  Distribution of biglycan and decorin in collateral and cruciate ligaments and menisci of the rabbit ...