Document Detail


Expression of Na+/HCO3- cotransporter and its role in pH regulation in mouse parotid acinar cells.
MedLine Citation:
PMID:  12727194     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ion transporters such as Na(+)/H(+) exchanger (NHE), Cl(-)/HCO(3)(-) exchanger (AE), and Na(+)/HCO(3)(-) cotransporter (NBC) are known to contribute to the intracellular pH (pH(i)) regulation during agonist-induced stimulation. This study examined the mechanisms for the pH(i) regulation in the mouse parotid and sublingual acinar cells using the fluorescent pH-sensitive probe, BCECF. The pH(i) recovery from agonist-induced acidification in the sublingual acinar cells was completely blocked by EIPA, a NHE inhibitor. However, the parotid acinar cells required DIDS, a NBC1 inhibitor, in addition to EIPA in order to block the pH(i) recovery. Moreover, RT-PCR analysis detected the expression of pancreatic NBC1 (pNBC1) only in the parotid acinar cells. These results provide strong evidence that the mechanisms for the pH(i) regulation are different in the two types of acinar cells, and pNBC1 contributes to pH(i) regulation in the parotid acinar cells, whereas NHE is likely to be the exclusive pH(i) regulator in the sublingual acinar cells.
Authors:
Youn-Bae Kim; Byung Ho Yang; Zhang Gen Piao; Seog Bae Oh; Joong Soo Kim; Kyungpyo Park
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  304     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2003 May 
Date Detail:
Created Date:  2003-05-02     Completed Date:  2003-06-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  593-8     Citation Subset:  IM    
Affiliation:
Department of Physiology, College of Dentistry and Dental Research Institute, Seoul National University, 28-2 Yeongeon-Dong Chongno-Ku, Seoul 110-749, Republic of Korea.
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MeSH Terms
Descriptor/Qualifier:
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / metabolism
Animals
Fluoresceins / metabolism
Fluorescent Dyes / metabolism
Hydrogen-Ion Concentration
Mice
Parotid Gland / cytology*,  metabolism*
Protein Isoforms / genetics,  metabolism
Receptors, Muscarinic / metabolism
Sodium-Bicarbonate Symporters / genetics,  metabolism*
Sodium-Hydrogen Antiporter / metabolism
Sublingual Gland / cytology,  metabolism
Chemical
Reg. No./Substance:
0/Fluoresceins; 0/Fluorescent Dyes; 0/Protein Isoforms; 0/Receptors, Muscarinic; 0/Slc4a4 protein, mouse; 0/Sodium-Bicarbonate Symporters; 0/Sodium-Hydrogen Antiporter; 53005-05-3/4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; 85138-49-4/2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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