Document Detail


Expression of a core 3 disialyl-Le(x) hexasaccharide in human colorectal cancers: a potential marker of malignant transformation in colon.
MedLine Citation:
PMID:  19152289     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cancer-associated alterations in cell surface and secreted glycoproteins have been catalogued for many years but many of the studies of alterations in mucin carbohydrate have relied on histochemical or immunohistochemical methods, with little direct chemical analysis. In this study, we analyzed the O-glycosylation pattern of MUC2 glycoprotein isolated from colorectal carcinomas, transitional mucosa and resection margins from three patients with blood group A, B and O, respectively. After alkaline borohydride treatment, the released oligosaccharides were structurally characterized by nanoESI Q-TOF tandem mass spectrometry without prior fractionation or derivatization. As expected, we found an increased expression of sialyl-Tn antigen in the colonic cancer mucins. A more interesting feature was the increased expression of a core 3 sialyl-Le(x) hexasaccharide, NeuAcalpha2-3Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-3(NeuAcalpha2-6)GalNAc in tumor, which appeared to compete with its sulfo-Le(x) counterpart in normal tissue, SO3-3Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-3(NeuAcalpha2-6)GalNAc. This antigen, whose structure was confirmed by NMR experiments, is based on a core 3 glycan and may be a potential marker for the malignant transformation of colonic cells. Unexpectedly, most of the glycans recovered in normal and carcinomas extracts were based on a sialylated core 3, GlcNAcbeta1-3(NeuAcalpha2-6)GalNAcol. Moreover, the pattern of glycosylation was very similar between mucins isolated from each sample, the main differences related to the level of expression of the major oligosaccharides. The data obtained in this investigation may have value for future screening studies on colorectal cancer.
Authors:
Catherine Robbe-Masselot; Annkatrin Herrmann; Emmanuel Maes; Ingemar Carlstedt; Jean-Claude Michalski; Calliope Capon
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of proteome research     Volume:  8     ISSN:  1535-3893     ISO Abbreviation:  J. Proteome Res.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-02-06     Completed Date:  2009-04-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101128775     Medline TA:  J Proteome Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  702-11     Citation Subset:  IM    
Affiliation:
Unite de Glycobiologie Structurale et Fonctionnelle, Universite des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq Cedex, France. catherine.robbe@univ-lille1.fr
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MeSH Terms
Descriptor/Qualifier:
Antigens, Tumor-Associated, Carbohydrate* / chemistry,  metabolism
Blood Group Antigens / chemistry,  metabolism
Carbohydrate Conformation
Carbohydrate Sequence
Cell Transformation, Neoplastic*
Colon / pathology*
Colorectal Neoplasms* / chemistry,  pathology
Humans
Mass Spectrometry
Molecular Sequence Data
Mucin-2* / chemistry,  metabolism
Mucins / chemistry
Oligosaccharides* / chemistry,  metabolism
Chemical
Reg. No./Substance:
0/Antigens, Tumor-Associated, Carbohydrate; 0/Blood Group Antigens; 0/Lewis a oligosaccharide; 0/MUC2 protein, human; 0/Mucin-2; 0/Mucins; 0/Oligosaccharides

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