Document Detail

Expression control and specificity of the basic amino acid exporter LysE of Corynebacterium glutamicum.
MedLine Citation:
PMID:  11429454     Owner:  NLM     Status:  MEDLINE    
LysE of Corynebacterium glutamicum belongs to a large new superfamily of translocators whose members are probably all involved in the export of small solutes. Here, the transcript initiation site of lysE, and its divergently transcribed regulator gene, lysG, are identified. Single-copy transcriptional fusions of lysE with lacZ, and titration experiments, show that LysG is the positive regulator of lysE expression enabling its up to 20-fold induction. This induction requires the presence of a coinducer, which is either intracellular L-lysine, or L-arginine. A competition experiment showed that LysE exports these two basic amino acids at comparable rates of about 0.75 nmol min(-1) (mg dry wt)(-1). Although L-histidine and L-citrulline also act as coinducers of lysE expression, these two amino acids are not exported by LysE. As is evident from the analysis of a lysEG deletion mutant, the physiological role of the lysEG system is to prevent bacteriostasis due to elevated L-lysine or L-arginine concentrations that arise during growth in the presence of peptides or in mutants possessing a deregulated biosynthesis pathway. C. glutamicum has additional export activities other than those of LysE for exporting L-histidine, L-citrulline and L-ornithine.
A Bellmann; M Vrljić; M Pátek; H Sahm; R Krämer; L Eggeling
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Microbiology (Reading, England)     Volume:  147     ISSN:  1350-0872     ISO Abbreviation:  Microbiology (Reading, Engl.)     Publication Date:  2001 Jul 
Date Detail:
Created Date:  2001-06-28     Completed Date:  2001-12-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9430468     Medline TA:  Microbiology     Country:  England    
Other Details:
Languages:  eng     Pagination:  1765-74     Citation Subset:  IM    
Institut für Biotechnologie, Forschungszentrum Jülich GmbH, D-52425 Jülich, Germany.
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MeSH Terms
Amino Acid Transport Systems, Basic*
Amino Acids, Diamino / metabolism*
Bacterial Proteins / genetics,  metabolism*
Carrier Proteins / genetics,  metabolism*
Corynebacterium / genetics*,  metabolism
Gene Expression Regulation, Bacterial*
Genes, Regulator
Substrate Specificity
Transcription, Genetic
Reg. No./Substance:
0/Amino Acid Transport Systems, Basic; 0/Amino Acids, Diamino; 0/Bacterial Proteins; 0/Carrier Proteins; 0/LysE protein, Corynebacterium glutamicum; 0/LysG protein, Corynebacterium glutamicum

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