Document Detail


Expression of collagenase-3 (matrix metalloproteinase-13) in squamous cell carcinomas of the head and neck.
MedLine Citation:
PMID:  9250162     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Squamous cell carcinomas (SCCs) of the head and neck are malignant tumors with high capacity to invade and metastasize. We have examined expression of the new collagenase, collagenase-3 (MMP-13), in SCCs of the head and neck. MMP-13 mRNAs were detected in 22 of 29 SCC cell lines: in 14 of 15 primary SCC cell lines and in 8 of 14 SCC cell lines from recurrent tumors or metastases. MMP-13 mRNAs were expressed by all 6 cell lines from highly invasive primary tumors and in all 4 cell lines from small aggressive tumors. Using in situ hybridization, MMP-13 mRNAs were detected in 15 of 17 SCC tumor samples. In most tumors, MMP-13 was expressed by tumor cells at the invading front of the tumors, but in a subset of SCCs, MMP-13 mRNA was also expressed by stromal fibroblasts. No MMP-13 expression was detected in intact skin or oral mucosa. MMP-13 mRNA levels in SCC cells were enhanced by transforming growth factor-beta, tumor necrosis factor-alpha, transforming growth factor-alpha, and keratinocyte growth factor. Specific expression of MMP-13 by SCC cells in vitro and in vivo strongly suggests a role for MMP-13 in the high invasion capacity of SCC cells.
Authors:
N Johansson; K Airola; R Grénman; A L Kariniemi; U Saarialho-Kere; V M Kähäri
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of pathology     Volume:  151     ISSN:  0002-9440     ISO Abbreviation:  Am. J. Pathol.     Publication Date:  1997 Aug 
Date Detail:
Created Date:  1997-08-26     Completed Date:  1997-08-26     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0370502     Medline TA:  Am J Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  499-508     Citation Subset:  AIM; IM    
Affiliation:
Department of Dermatology, Turku University Central Hospital, Finland.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Aged, 80 and over
Carcinoma, Squamous Cell / enzymology*
Collagenases / analysis,  biosynthesis*
Female
Head and Neck Neoplasms / enzymology*
Humans
In Situ Hybridization
Male
Matrix Metalloproteinase 13
Middle Aged
RNA, Messenger / analysis
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/RNA, Messenger; EC 3.4.24.-/Collagenases; EC 3.4.24.-/MMP13 protein, human; EC 3.4.24.-/Matrix Metalloproteinase 13
Comments/Corrections

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