| Expression of clusterin in cell differentiation and cell death. | |
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MedLine Citation:
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PMID: 7654325 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Clusterin, originally isolated as testosterone-repressed prostate message-2 from regressing rat ventral prostate, has been identified with the process of active cell death (ACD). The clusterin gene product is a glycosylated dimer consisting of alpha and beta subunits, resulting from the 70-kilodalton preprotein. To determine its relationship with ACD, we have examined clusterin expression via in situ hybridization and immunohistochemistry. Clusterin message is detected in the supporting cells in both testes and ovaries and the protein surrounds the mature germ cells. The highest level of expression was found in the head region of the epididymis. Clusterin message is also detected in selected cells of uterine glands and ducts both in the normal and pregnant uterus. The expression of clusterin in the developing embryo is most abundant in the choroid plexus, inner ear, and epithelium of the eye. None of the cells in the testes, epididymis, or embryo that express clusterin are undergoing ACD. The expression of clusterin appears to correlate with cell remodelling or differentiation that occurs during these periods of development. However, in the female reproductive system, we found clusterin to be expressed in both differentiating as well as dying cells. These results suggest that clusterin may provide support for cells undergoing specific biochemical and (or) physical changes. Our results are consistent with the hypothesis that clusterin is an antiinflammatory agent. |
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Authors:
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H S Ahuja; M Tenniswood; R Lockshin; Z F Zakeri |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochemistry and cell biology = Biochimie et biologie cellulaire Volume: 72 ISSN: 0829-8211 ISO Abbreviation: Biochem. Cell Biol. Publication Date: 1994 Nov-Dec |
Date Detail:
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Created Date: 1995-10-05 Completed Date: 1995-10-05 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8606068 Medline TA: Biochem Cell Biol Country: CANADA |
Other Details:
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Languages: eng Pagination: 523-30 Citation Subset: IM |
Affiliation:
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Queens College, Department of Biology, City University of New York, Flushing 11367, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis* Cell Differentiation Clusterin Embryonic and Fetal Development Female Glycoproteins / biosynthesis* Male Mice Molecular Chaperones* Ovary / embryology, metabolism* Pregnancy RNA, Messenger / analysis Testis / embryology, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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1K04AG00631-1/AG/NIA NIH HHS; R01-01-01//PHS HHS |
| Chemical | |
Reg. No./Substance:
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0/Clu protein, mouse; 0/Clusterin; 0/Glycoproteins; 0/Molecular Chaperones; 0/RNA, Messenger |
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