Document Detail


Expression of clusterin in Crohn's disease of the terminal ileum.
MedLine Citation:
PMID:  11510965     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Crohn's disease (CD) is a chronic inflammatory intestinal disorder with disturbance and injury of the intestinal mucosal barrier, in which various proinflammatory molecules as well as molecules with antiinflammatory activity and cytoprotective function are found to be expressed. We investigated whether clusterin, a multifunctional cytoprotective protein, is upregulated in Crohn's disease, because augmented expression of clusterin is seen in many organs following various forms of tissue injury. Human actively and inactively inflamed ileal tissues from CD patients as well as normal intestinal specimens from control patients (normal ileum) were investigated by Western blot analysis, immunohistochemisty and in situ hybridization. As compared with controls, a strongly enhanced expression of clusterin was found in CD tissues, correlating with disease activity. Immunohistochemistry and in situ hybridization analysis revealed foci of crypts almost completely lined by clusterin expressing enterocytes in CD, a feature that was never seen in controls. Such crypts appeared especially within the morphologically intact mucosa apart from erosive or ulcerative lesions. Besides epithelia, clusterin was also expressed by inflammatory mononuclear cells. Enhanced expression of clusterin by crypt epithelia might reflect a cytoprotective function of the protein in order to prevent further injury of the intestinal mucosal barrier in CD.
Authors:
N Gassler; F Autschbach; G Heuschen; R Witzgall; H F Otto; N Obermüller
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Histology and histopathology     Volume:  16     ISSN:  0213-3911     ISO Abbreviation:  Histol. Histopathol.     Publication Date:  2001 Jul 
Date Detail:
Created Date:  2001-08-20     Completed Date:  2002-02-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8609357     Medline TA:  Histol Histopathol     Country:  Spain    
Other Details:
Languages:  eng     Pagination:  755-62     Citation Subset:  IM    
Affiliation:
Institute of Pathology, University of Heidelberg, Germany. Nikolaus_Gassler@med.uni-heidelberg.de
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Blotting, Western
Case-Control Studies
Clusterin
Crohn Disease / genetics,  metabolism*,  pathology
Glycoproteins / genetics,  metabolism*
Humans
Ileum / metabolism*,  pathology
Immunohistochemistry
In Situ Hybridization
Intestinal Mucosa / metabolism,  pathology
Middle Aged
Molecular Chaperones / genetics,  metabolism*
RNA, Messenger / genetics,  metabolism
Up-Regulation
Chemical
Reg. No./Substance:
0/CLU protein, human; 0/Clusterin; 0/Glycoproteins; 0/Molecular Chaperones; 0/RNA, Messenger

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