| Expression of a cloned ovine gastrointestinal peptide transporter (oPepT1) in Xenopus oocytes induces uptake of oligopeptides in vitro. | |
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MedLine Citation:
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PMID: 11285336 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We determined the primary structure, tissue distribution and in vitro functional characterization of a peptide transporter, oPepT1, from ovine intestine. Ovine PepT1 (oPepT1) cDNA was 2829-bp long, encoding a protein of 707 amino acid residues with an estimated molecular size of 78 kDa and an isoelectric point (pI) of 6.57. Transport function of oPepT1 was assessed by expressing oPepT1 in Xenopus oocytes using a two-electrode voltage-clamp technique. The transport process was electrogenic and pH dependent, but independent of Na+, Cl- and Ca2+. The oPepT1 displayed a broad substrate specificity for transport of neutral and charged dipeptides and tripeptides. All dipeptides and tripeptides examined evoked inward currents in a saturable manner, with an affinity constant (Kt) ranging from 27 micromol/L to 3.0 mmol/L. No responses were detected from tetrapeptides or free amino acids. Northern blot analysis demonstrated that oPepT1 was expressed in the small intestine, omasum and rumen, but was not expressed in liver and kidney. The presence of the peptide transporter in the forestomach at such levels could provide nutritionally important amino acid nitrogen to ruminants. |
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Authors:
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Y Pan; E A Wong; J R Bloomquist; K E Webb |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of nutrition Volume: 131 ISSN: 0022-3166 ISO Abbreviation: J. Nutr. Publication Date: 2001 Apr |
Date Detail:
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Created Date: 2001-04-04 Completed Date: 2001-05-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0404243 Medline TA: J Nutr Country: United States |
Other Details:
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Languages: eng Pagination: 1264-70 Citation Subset: IM |
Affiliation:
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Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence
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genetics Animals Carrier Proteins / genetics, pharmacology* Cells, Cultured Cloning, Molecular DNA, Complementary / genetics Dipeptides / pharmacology Electric Conductivity Female Gene Expression Ions Molecular Sequence Data Oligopeptides / pharmacokinetics* Oocytes / metabolism*, physiology Osmolar Concentration Perfusion RNA, Messenger / metabolism Sheep Substrate Specificity Symporters* Tissue Distribution |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/DNA, Complementary; 0/Dipeptides; 0/Ions; 0/Oligopeptides; 0/PepT1 protein; 0/RNA, Messenger; 0/Symporters |
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