Document Detail


Expression of a cloned ovine gastrointestinal peptide transporter (oPepT1) in Xenopus oocytes induces uptake of oligopeptides in vitro.
MedLine Citation:
PMID:  11285336     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We determined the primary structure, tissue distribution and in vitro functional characterization of a peptide transporter, oPepT1, from ovine intestine. Ovine PepT1 (oPepT1) cDNA was 2829-bp long, encoding a protein of 707 amino acid residues with an estimated molecular size of 78 kDa and an isoelectric point (pI) of 6.57. Transport function of oPepT1 was assessed by expressing oPepT1 in Xenopus oocytes using a two-electrode voltage-clamp technique. The transport process was electrogenic and pH dependent, but independent of Na+, Cl- and Ca2+. The oPepT1 displayed a broad substrate specificity for transport of neutral and charged dipeptides and tripeptides. All dipeptides and tripeptides examined evoked inward currents in a saturable manner, with an affinity constant (Kt) ranging from 27 micromol/L to 3.0 mmol/L. No responses were detected from tetrapeptides or free amino acids. Northern blot analysis demonstrated that oPepT1 was expressed in the small intestine, omasum and rumen, but was not expressed in liver and kidney. The presence of the peptide transporter in the forestomach at such levels could provide nutritionally important amino acid nitrogen to ruminants.
Authors:
Y Pan; E A Wong; J R Bloomquist; K E Webb
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of nutrition     Volume:  131     ISSN:  0022-3166     ISO Abbreviation:  J. Nutr.     Publication Date:  2001 Apr 
Date Detail:
Created Date:  2001-04-04     Completed Date:  2001-05-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0404243     Medline TA:  J Nutr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1264-70     Citation Subset:  IM    
Affiliation:
Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence / genetics
Animals
Carrier Proteins / genetics,  pharmacology*
Cells, Cultured
Cloning, Molecular
DNA, Complementary / genetics
Dipeptides / pharmacology
Electric Conductivity
Female
Gene Expression
Ions
Molecular Sequence Data
Oligopeptides / pharmacokinetics*
Oocytes / metabolism*,  physiology
Osmolar Concentration
Perfusion
RNA, Messenger / metabolism
Sheep
Substrate Specificity
Symporters*
Tissue Distribution
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/DNA, Complementary; 0/Dipeptides; 0/Ions; 0/Oligopeptides; 0/PepT1 protein; 0/RNA, Messenger; 0/Symporters

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