Document Detail

Expression of ceramide glucosyltransferases, which are essential for glycosphingolipid synthesis, is only required in a small subset of C. elegans cells.
MedLine Citation:
PMID:  19240113     Owner:  NLM     Status:  MEDLINE    
Glycosphingolipids (GSLs) are glycosylated derivatives of ceramide in the lipid bilayer. Their ubiquitous distribution and complexity suggest that they have important functions, but what these are in vivo is still poorly understood. Here, we characterize the phenotype of Caenorhabditis elegans mutants with essentially no GSLs. The C. elegans genome encodes three ceramide glucosyltransferase (CGT) genes, which encode enzymes required for GSL biosynthesis. Animals lacking CGT do not synthesize GSLs, arrest growth at the first larval stage, and display defects in a subset of cells in their digestive tract; these defects impair larval feeding, resulting in a starvation-induced growth arrest. Restoring CGT function in these digestive tract cells - but not in a variety of other tissues - is sufficient to rescue the phenotypes associated with loss of CGT function. These unexpected findings suggest that GSLs are dispensable in most C. elegans cells, including those of the nervous system.
Esther Marza; Karina T Simonsen; Nils J Faergeman; Giovanni M Lesa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-02-24
Journal Detail:
Title:  Journal of cell science     Volume:  122     ISSN:  0021-9533     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-03-05     Completed Date:  2009-06-15     Revised Date:  2014-02-19    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  822-33     Citation Subset:  IM    
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MeSH Terms
Amino Acid Sequence
Caenorhabditis elegans / cytology*,  embryology,  enzymology*,  ultrastructure
Cell Proliferation
Cell Shape
Ceramides / metabolism
Embryo, Nonmammalian / cytology,  enzymology
Feeding Behavior
Gene Expression Regulation, Developmental
Gene Expression Regulation, Enzymologic
Gene Knockout Techniques
Genes, Helminth
Glucosyltransferases / chemistry,  genetics*
Glycosphingolipids / biosynthesis*,  chemistry
Larva / enzymology,  genetics
Molecular Sequence Data
Mutation / genetics
Nervous System / enzymology
Organ Specificity
RNA, Messenger / genetics,  metabolism
Transformation, Genetic
Grant Support
MC_U122663296//Medical Research Council; //Cancer Research UK; //Medical Research Council
Reg. No./Substance:
0/Ceramides; 0/Glycosphingolipids; 0/RNA, Messenger; EC 2.4.1.-/Glucosyltransferases; EC glucosyltransferase
Erratum In:
J Cell Sci. 2009 May 15;122(Pt 10):1700

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