Document Detail


Expression and cell type--specific processing of human preproenkephalin with a vaccinia recombinant.
MedLine Citation:
PMID:  3754979     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The posttranslational maturation of a complex precursor polyprotein, human proenkephalin, was assessed by infection of a wide spectrum of cell types with a recombinant vaccinia virus that expressed human proenkephalin. The infected cells rapidly produced both cellular and secreted Met-enkephalin immunoreactivity. Although each cell line could secrete intact proenkephalin, only a mouse pituitary line was capable of processing proenkephalin to mature enkephalin peptides. The quantity of intact proenkephalin secreted from BSC-40 cells (derived from African Green monkey kidney) was sufficient to establish the value of vaccinia virus as a mammalian cell expression vector.
Authors:
G Thomas; E Herbert; D E Hruby
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Science (New York, N.Y.)     Volume:  232     ISSN:  0036-8075     ISO Abbreviation:  Science     Publication Date:  1986 Jun 
Date Detail:
Created Date:  1986-07-08     Completed Date:  1986-07-08     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0404511     Medline TA:  Science     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1641-3     Citation Subset:  IM    
Data Bank Information
Bank Name/Acc. No.:
GENBANK/M13459
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Cercopithecus aethiops
Enkephalin, Methionine / biosynthesis
Enkephalins / biosynthesis*,  genetics
Genetic Vectors
Humans
Mice
Protein Precursors / biosynthesis*,  genetics
Rats
Recombinant Proteins / biosynthesis*
Vaccinia virus / genetics*
Grant Support
ID/Acronym/Agency:
7 RO1 DA04154-01/DA/NIDA NIH HHS
Chemical
Reg. No./Substance:
0/Enkephalins; 0/Protein Precursors; 0/Recombinant Proteins; 0/proenkephalin; 58569-55-4/Enkephalin, Methionine; 93443-35-7/preproenkephalin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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