Document Detail

Expression of the cell proliferation-associated nuclear antigen reactive with the Ki-67 monoclonal antibody by cells of the skeletal system in humans and other species.
MedLine Citation:
PMID:  2397326     Owner:  NLM     Status:  MEDLINE    
The cell proliferation-associated nuclear antigen recognized by the Ki-67 monoclonal antibody was detected immunohistochemically in cells from skeletal tissues of humans, sheep and rabbits. In each case the distribution of Ki-67-positive cells was identical and consistent with the known distribution of dividing cells in growing long bones. The antigen recognized by Ki-67 is detectable only after fixation in cold acetone. In the 5-day-old rabbit, labelling with tritiated thymidine [( 3H]TdR) in vivo, followed subsequently by a combination of Ki-67 immunohistochemistry and autoradiography for [3H]TdR, demonstrated that, in addition to the expression of Ki-67 by the majority of [3H]TdR labelled cells, other cells associated with proliferating populations also expressed the Ki-67 antigen. This suggests that the Ki-67 antibody identifies cycling cells in the skeleton in S-phase, as well as in other phases of the cell cycle. In Ki-67 reactive species, this method is a potentially useful tool for the study of cell proliferation in skeletal tissues in vivo and in vitro, having the advantage of not requiring prior labelling with DNA precursors.
S S Apte
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Bone and mineral     Volume:  10     ISSN:  0169-6009     ISO Abbreviation:  Bone Miner     Publication Date:  1990 Jul 
Date Detail:
Created Date:  1990-10-18     Completed Date:  1990-10-18     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8610542     Medline TA:  Bone Miner     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  37-50     Citation Subset:  IM    
Nuffield Department of Orthopaedic Surgery, University of Oxford, Headington, England.
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MeSH Terms
Antibodies, Monoclonal*
Antigens, Surface / analysis*
Bone Development*
Bone Marrow / immunology
Bone Marrow Cells
Bone and Bones / cytology*,  embryology,  immunology
Cartilage / immunology
Cell Division
Cold Temperature
DNA / biosynthesis
Gestational Age
Growth Plate / cytology,  immunology
Ki-67 Antigen
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, Surface; 0/Fixatives; 0/Ki-67 Antigen; 67-64-1/Acetone; 9007-49-2/DNA

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