Document Detail


Expression of cell cycle-dependent genes in young and senescent WI-38 fibroblasts.
MedLine Citation:
PMID:  3458185     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We studied the expression of 11 cell cycle-dependent genes in senescent WI-38 fibroblasts and compared the results to those obtained in WI-38 cells from early passages (young cells). Every gene we examined is expressed in the senescent cells at levels similar to those in the young cells, including two genes maximally expressed at the G1/S phase boundary--genes for thymidine kinase and histone H3. The results clearly show that senescent, noncycling WI-38 cells are not similar to quiescent cells. Rather, such senescent WI-38 cells may be blocked just prior to the onset of DNA synthesis.
Authors:
S R Rittling; K M Brooks; V J Cristofalo; R Baserga
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  83     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1986 May 
Date Detail:
Created Date:  1986-06-23     Completed Date:  1986-06-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3316-20     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Cycle*
Cell Line
Cell Survival*
DNA Replication
Gene Expression Regulation
Histones / genetics
Humans
Interphase
Proto-Oncogenes
Thymidine Kinase / genetics
Grant Support
ID/Acronym/Agency:
AG-00378/AG/NIA NIH HHS
Chemical
Reg. No./Substance:
0/Histones; EC 2.7.1.21/Thymidine Kinase
Comments/Corrections

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