Document Detail

Expression of caveolin-1 enhances cholesterol efflux in hepatic cells.
MedLine Citation:
PMID:  14729661     Owner:  NLM     Status:  MEDLINE    
HepG2 cells were stably transfected with human caveolin-1 (HepG2/cav cells). Transfection resulted in expression of caveolin-1 mRNA, a high abundance of caveolin-1 protein, and the formation of caveolae on the plasma membrane. Cholesterol efflux from HepG2/cav cells was 280 and 45% higher than that from parent HepG2 cells when human plasma and human apoA-I, respectively, were used as acceptors. The difference in efflux was eliminated by treatment of cells with progesterone. There was no difference in cholesterol efflux to cyclodextrin. Cholesterol efflux from plasma membrane vesicles was similar for the two cell types. Transfection led to a 40% increase in the amount of plasma membrane cholesterol in cholesterol-rich domains (caveolae and/or rafts) and a 67% increase in the rate of cholesterol trafficking from intracellular compartments to these domains. Cholesterol biosynthesis in HepG2/cav cells was increased by 2-fold, and cholesterol esterification was reduced by 50% compared with parent HepG2 cells. The proliferation rate of transfected cells was significantly lower than that of non-transfected cells. Transfection did not affect expression of ABCA1 or the abundance of ABCA1 protein, but decreased secretion of apoA-I. We conclude that overexpression of caveolin-1 in hepatic cells stimulates cholesterol efflux by enhancing transfer of cholesterol to cholesterol-rich domains in the plasma membrane.
Ying Fu; Anh Hoang; Genevieve Escher; Robert G Parton; Zygmunt Krozowski; Dmitri Sviridov
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-01-16
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  279     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-03-29     Completed Date:  2004-05-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  14140-6     Citation Subset:  IM    
Wynn Domain, Baker Heart Research Institute, Melbourne, Victoria 8008, Australia.
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MeSH Terms
ATP-Binding Cassette Transporters / genetics
Carcinoma, Hepatocellular
Caveolin 1
Caveolins / genetics*,  metabolism*
Cell Division
Cell Line, Tumor
Cell Membrane / metabolism
Cholesterol / biosynthesis,  metabolism*
Gene Expression
Liver / cytology*
Reg. No./Substance:
0/ATP binding cassette transporter 1; 0/ATP-Binding Cassette Transporters; 0/CAV1 protein, human; 0/Caveolin 1; 0/Caveolins; 57-88-5/Cholesterol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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