| Expression of carboxylesterase and lipase genes in rat liver cell-types. | |
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MedLine Citation:
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PMID: 18639528 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Approximately 80% of the body vitamin A is stored in liver stellate cells with in the lipid droplets as retinyl esters. In low vitamin A status or after liver injury, stellate cells are depleted of the stored retinyl esters by their hydrolysis to retinol. However, the identity of retinyl ester hydrolase(s) expressed in stellate cells is unknown. The expression of carboxylesterase and lipase genes in purified liver cell-types was investigated by real-time PCR. We found that six carboxylesterase and hepatic lipase genes were expressed in hepatocytes. Adipose triglyceride lipase was expressed in Kupffer cells, stellate cells and endothelial cells. Lipoprotein lipase expression was detected in Kupffer cells and stellate cells. As a function of stellate cell activation, expression of adipose triglyceride lipase decreased by twofold and lipoprotein lipase increased by 32-fold suggesting that it may play a role in retinol ester hydrolysis during stellate cell activation. |
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Authors:
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Tommaso Mello; Alice Nakatsuka; Sharry Fears; Wilhelmina Davis; Hidekazu Tsukamoto; William F Bosron; Sonal P Sanghani |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2008-07-17 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 374 ISSN: 1090-2104 ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2008 Sep |
Date Detail:
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Created Date: 2008-08-15 Completed Date: 2008-09-05 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 460-4 Citation Subset: IM |
Affiliation:
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Gastroenterology Unit, Department of Clinical Pathophysiology, University of Florence, Florence, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carboxylic Ester Hydrolases / genetics* Cell Separation Cells, Cultured Endothelial Cells / enzymology Gene Expression* Hepatocytes / enzymology* Kupffer Cells / enzymology Lipase / genetics* Lipoprotein Lipase / genetics Liver / cytology, enzymology* Polymerase Chain Reaction Rats Vitamin A / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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R01 DK063141-01A1/DK/NIDDK NIH HHS; R01DK063141/DK/NIDDK NIH HHS; R21 AA012413-01/AA/NIAAA NIH HHS |
| Chemical | |
Reg. No./Substance:
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11103-57-4/Vitamin A; EC 3.1.1.-/Carboxylic Ester Hydrolases; EC 3.1.1.-/retinyl esterase; EC 3.1.1.3/Lipase; EC 3.1.1.34/Lipoprotein Lipase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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