Document Detail

Expression of carboxylesterase and lipase genes in rat liver cell-types.
MedLine Citation:
PMID:  18639528     Owner:  NLM     Status:  MEDLINE    
Approximately 80% of the body vitamin A is stored in liver stellate cells with in the lipid droplets as retinyl esters. In low vitamin A status or after liver injury, stellate cells are depleted of the stored retinyl esters by their hydrolysis to retinol. However, the identity of retinyl ester hydrolase(s) expressed in stellate cells is unknown. The expression of carboxylesterase and lipase genes in purified liver cell-types was investigated by real-time PCR. We found that six carboxylesterase and hepatic lipase genes were expressed in hepatocytes. Adipose triglyceride lipase was expressed in Kupffer cells, stellate cells and endothelial cells. Lipoprotein lipase expression was detected in Kupffer cells and stellate cells. As a function of stellate cell activation, expression of adipose triglyceride lipase decreased by twofold and lipoprotein lipase increased by 32-fold suggesting that it may play a role in retinol ester hydrolysis during stellate cell activation.
Tommaso Mello; Alice Nakatsuka; Sharry Fears; Wilhelmina Davis; Hidekazu Tsukamoto; William F Bosron; Sonal P Sanghani
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2008-07-17
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  374     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-08-15     Completed Date:  2008-09-05     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  460-4     Citation Subset:  IM    
Gastroenterology Unit, Department of Clinical Pathophysiology, University of Florence, Florence, Italy.
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MeSH Terms
Carboxylic Ester Hydrolases / genetics*
Cell Separation
Cells, Cultured
Endothelial Cells / enzymology
Gene Expression*
Hepatocytes / enzymology*
Kupffer Cells / enzymology
Lipase / genetics*
Lipoprotein Lipase / genetics
Liver / cytology,  enzymology*
Polymerase Chain Reaction
Vitamin A / metabolism*
Grant Support
R01 DK063141-01A1/DK/NIDDK NIH HHS; R01DK063141/DK/NIDDK NIH HHS; R21 AA012413-01/AA/NIAAA NIH HHS
Reg. No./Substance:
11103-57-4/Vitamin A; EC 3.1.1.-/Carboxylic Ester Hydrolases; EC 3.1.1.-/retinyl esterase; EC; EC Lipase

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