Document Detail

Expression of carbonic anhydrase-related protein CA-RP VIII in non-small cell lung cancer.
MedLine Citation:
PMID:  12536316     Owner:  NLM     Status:  MEDLINE    
Carbonic anhydrase-related protein (CA-RP VIII) lacks a Zn-binding motif which is essential for carbonic anhydrase activity. Therefore, CA-RP VIII is believed to have a no catalytic activity and a new biological property. In the present study, CA-RP VIII expression in non-tumorous lung and non-small cell lung carcinomas was investigated. Little or no expression of CA-RP VIII was observed in human lungs by Northern-blot analysis. Reverse-transcription polymerase chain reaction analysis demonstrated CA-RP VIII mRNA in developing human lungs and, to a lesser extent, in normal lungs. Subsequent immunohistochemical staining revealed that CA-RP VIII was expressed in the pulmonary epithelium in developing lungs; however, CA-RP VIII expression was restricted in bronchial ciliated cells in adult lungs. Neither bronchial gland nor squamous metaplasia of interstitial pneumonia expressed CA-RP VIII. In contrast, CA-RP VIII was strongly expressed in almost all archival lung cancer specimens, which included 24 squamous cell carcinomas, 25 adenocarcinomas, and 6 adenosquamous cell carcinomas. Cancer cells at the front of tumor progression expressed CA-RP VIII in particular abundance. The present findings suggest that CA-RP VIII may play a role in non-small lung cell carcinomas.
Yousuke Akisawa; Isao Nishimori; Keisuke Taniuchi; Nobuto Okamoto; Tamotsu Takeuchi; Hiroshi Sonobe; Yuji Ohtsuki; Saburo Onishi
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Publication Detail:
Type:  Journal Article     Date:  2002-12-05
Journal Detail:
Title:  Virchows Archiv : an international journal of pathology     Volume:  442     ISSN:  0945-6317     ISO Abbreviation:  Virchows Arch.     Publication Date:  2003 Jan 
Date Detail:
Created Date:  2003-01-21     Completed Date:  2003-03-04     Revised Date:  2006-11-20    
Medline Journal Info:
Nlm Unique ID:  9423843     Medline TA:  Virchows Arch     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  66-70     Citation Subset:  IM    
First Department of Internal Medicine, Kochi Medical School, Nankoku, Kochi 783-8505, Japan.
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MeSH Terms
Blotting, Northern
Bronchi / enzymology,  pathology
Carbonic Anhydrases*
Carcinoma, Non-Small-Cell Lung / enzymology*,  pathology
Cell Count
DNA Primers / chemistry
Epithelial Cells / enzymology,  pathology
Lung / embryology,  enzymology,  pathology
Lung Neoplasms / enzymology*,  pathology
Mice, Inbred BALB C
Nerve Tissue Proteins / biosynthesis*,  genetics
RNA, Messenger / biosynthesis,  genetics
RNA, Neoplasm / analysis
Reverse Transcriptase Polymerase Chain Reaction
Tumor Markers, Biological
Reg. No./Substance:
0/CA8 protein, human; 0/Car8 protein, mouse; 0/DNA Primers; 0/Nerve Tissue Proteins; 0/RNA, Messenger; 0/RNA, Neoplasm; 0/Tumor Markers, Biological; EC Anhydrases

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