Document Detail

Expression of cAMP response element-binding protein in the duct system of the mouse submandibular gland.
MedLine Citation:
PMID:  19838724     Owner:  NLM     Status:  MEDLINE    
The submandibular gland (SMG) of mice shows a marked sexual dimorphism in which a duct portion called the granular convoluted tubule (GCT) is developed preferentially in males during puberty. The administration of testosterone to female mice causes the conversion of striated duct (SD) cells into GCT cells, but the underlying molecular mechanisms are unclear. Cyclic AMP response element-binding protein (CREB) is a transcription factor functioning downstream of a variety of signal transduction pathways. In the present study, we examined the expression, activation and cellular localization of CREB in the mouse SMG using Western blotting and immunohistochemistry. Both total CREB (t-CREB) and phosphorylated CREB (p-CREB) were significantly more abundant in the female than in the male gland and were localized to the nuclei of intercalated duct cells and a subpopulation of SD cells. In contrast, the GCT cells in males were negative for t- and p-CREB. The levels of CREB in the SMG were increased by castration in males and decreased by repeated administration of testosterone to females or castrated males. From 3 h after a single administration of testosterone to females, many SD cells temporarily gained nuclear immunoreactivity for both t- and p-CREB, which was lost as the cells were converted to GCT cells by 24 h. These results suggest the involvement of CREB in the androgen-dependent differentiation of the duct system in the mouse SMG.
Sunisa Keattikunpairoj; Tomohiko Wakayama; Miyuki Yamamoto; Masa-aki Nakaya; Hiroki Nakata; Wiphawi Hipkaeo; Natthiya Sakulsak; Shoichi Iseki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-10-17
Journal Detail:
Title:  Histochemistry and cell biology     Volume:  132     ISSN:  1432-119X     ISO Abbreviation:  Histochem. Cell Biol.     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2010-01-21     Completed Date:  2010-04-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9506663     Medline TA:  Histochem Cell Biol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  647-57     Citation Subset:  IM    
Department of Histology and Embryology, Graduate School of Medical Science, Kanazawa University, 13-1 Takara-machi, Kanazawa, 920-8640, Japan.
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MeSH Terms
Cyclic AMP Response Element-Binding Protein / analysis*
Sex Characteristics*
Submandibular Gland / chemistry*
Testosterone / pharmacology
Reg. No./Substance:
0/Cyclic AMP Response Element-Binding Protein; 58-22-0/Testosterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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