Document Detail


Expression of c-fos and c-jun protooncogenes in the uteri of immature mice neonatally exposed to diethylstilbestrol.
MedLine Citation:
PMID:  12507287     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We studied the cell-type-specific and temporal expression of c-fos and c-jun protooncogenes after 17beta-estradiol (E2) stimulation in the uteri of immature 3-week-old mice neonatally exposed to diethylstilbestrol (DES), DES-mice, and the ontogenic expression of these genes in the uteri of DES-mice using immunohistochemistry and in situ hybridization. A single E2 injection induced the transient and rapid expression of c-fos mRNA and c-Fos protein in the endometrial epithelium and endothelial cells of the blood vessels in both 3-week-old vehicle-treated controls and DES-mice; a peak of mRNA expression was 2 hours after E2 injection and that of protein expression was 2 to 3 hours after the injection. The expression of c-fos mRNA and protein after E2 stimulation was lower in the DES-mice than in the control animals. There were no significant differences in the c-jun expression patterns in both experimental groups before and after the E2 injection. The E2 injection transiently down-regulated the c-jun expression in the epithelium and up-regulated it in the stroma and myometrium. The uterine epithelium of DES-mice showed much stronger c-Jun immunostaining on days 4 and 10, compared with those of controls. Neonatal DES treatment reduced c-Jun immunoreactivity in the uterine epithelium on days 4 and 10, and increased the reaction in the stroma on day 4. These results suggested that the neonatal DES treatment induces permanent changes in the c-fos expression pattern independent of the postpuberal secretion of ovarian steroids. The changes in the expression of c-fos and c-jun protooncogenes, particularly during postnatal development, are likely to play important roles in the production of uterine abnormalities in the DES-mice.
Authors:
S Yamashita; A Takayanagi; N Shimizu
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Histology and histopathology     Volume:  18     ISSN:  0213-3911     ISO Abbreviation:  Histol. Histopathol.     Publication Date:  2003 Jan 
Date Detail:
Created Date:  2002-12-31     Completed Date:  2003-06-05     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8609357     Medline TA:  Histol Histopathol     Country:  Spain    
Other Details:
Languages:  eng     Pagination:  83-92     Citation Subset:  IM    
Affiliation:
Keio Junior College of Nursing, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan. shuji@sc.itc.keio.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Diethylstilbestrol / pharmacology*
Estrogens, Non-Steroidal / pharmacology*
Female
Genes, fos*
Genes, jun*
Immunohistochemistry
In Situ Hybridization
Mice
Proto-Oncogene Proteins c-fos / metabolism
Proto-Oncogene Proteins c-jun / metabolism
Uterus / drug effects*,  growth & development,  metabolism*
Chemical
Reg. No./Substance:
0/Estrogens, Non-Steroidal; 0/Proto-Oncogene Proteins c-fos; 0/Proto-Oncogene Proteins c-jun; 56-53-1/Diethylstilbestrol

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