Document Detail


Expression of c-fos, c-jun and HSP70 mRNA in rat brain following high acceleration stress.
MedLine Citation:
PMID:  11539307     Owner:  NASA     Status:  MEDLINE    
Abstract/OtherAbstract:
Rats exposed to high +Gz forces in a small animal centrifuge (SAC) exhibit loss of neuronal function (isoelectric EEG), termed G-induced loss of consciousness (G-LOC). This phenomenon is presumably due to a reduction in cerebral blood flow (CBF) or ischemia. Ischemia induces various metabolic and physiologic changes including expression of immediate early genes (IEGs) in the brain. Expression of IEGs have been suggested to be reliable markers for neuronal response to external stimuli or stress. In the present study expression of IEGs c-fos, c-jun and stress response gene HSP70 were measured in the brains of rats subjected to six 30 s exposures of +22.5Gz in a small animal centrifuge. The level of c-fos, HSP70 and beta-actin mRNA were measured by both Northern blot and RT-PCR. Expression of c-jun was measured only by RT-PCR. Expression of c-fos and c-jun was significantly stimulated at 0.5, 15, 30 and 60 min post-centrifugation. The level of HSP70 mRNA was significantly higher only at 60 and 180 min post-centrifugation. Measurement of metabolities showed a significant increase in lactate and a decrease in Cr-P level at 30 s and 15 min post-centrifugation, respectively. Lactate, but not Cr-P and ATP levels were restored to control levels by 60 min post-centrifugation. It is concluded that the transient expression of c-fos, c-jun and HSP70 mRNA is stimulated by repeated ischemic/reperfusion episodes induced by high acceleration stress.
Authors:
A R Shahed; M Son; J C Lee; P M Werchan
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of gravitational physiology : a journal of the International Society for Gravitational Physiology     Volume:  3     ISSN:  1077-9248     ISO Abbreviation:  J Gravit Physiol     Publication Date:  1996 Apr 
Date Detail:
Created Date:  1997-06-29     Completed Date:  1997-06-29     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9437868     Medline TA:  J Gravit Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  49-56     Citation Subset:  S    
Affiliation:
Operational Technologies Corporation.
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MeSH Terms
Descriptor/Qualifier:
Acceleration / adverse effects*
Animals
Brain / metabolism,  physiopathology*
Brain Ischemia / etiology,  genetics,  physiopathology
Centrifugation
Gene Expression*
Genes, Immediate-Early / genetics,  physiology
Genes, fos / genetics,  physiology*
Genes, jun / genetics,  physiology*
HSP70 Heat-Shock Proteins / genetics,  metabolism*
Hypergravity / adverse effects*
Male
Rats
Rats, Sprague-Dawley
Stress, Physiological / etiology,  genetics,  physiopathology
Chemical
Reg. No./Substance:
0/HSP70 Heat-Shock Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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