Document Detail


Expression of both B7-1 and CD28 contributes to the IL-2 responsiveness of CTLL-2 cells.
MedLine Citation:
PMID:  8698390     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The CTLL-2 bioassay is used frequently to determine interleukin-2 (IL-2) concentrations in experimental samples, including samples that contain reagents which affect the CD28-B7 interaction. We therefore evaluated whether the CD28-B7 pathway plays a role in the growth of CTLL-2 cells. Flow cytometry demonstrated that CTLL-2 cells express both CD28 and B7-1. CTLA4-immunoglobulin (CTLA4-Ig) inhibited the growth of CTLL-2 cells over a range of IL-2 concentrations, suggesting that the CD28-B7 interaction plays an important role in the growth of CTLL-2 cells. Anti-B7-1 antibody also inhibited CTLL-2 proliferation at all concentrations of IL-2. These results indicate that the CTLL-2 bioassay may not be a reliable means of determining IL-2 levels in experimental samples containing reagents that affect the CD28-B7 interaction. They also suggest that co-expression of CD28 and B7 may contribute to the growth of malignant T cells.
Authors:
R Belani; G J Weiner
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Immunology     Volume:  87     ISSN:  0019-2805     ISO Abbreviation:  Immunology     Publication Date:  1996 Feb 
Date Detail:
Created Date:  1996-09-05     Completed Date:  1996-09-05     Revised Date:  2013-05-24    
Medline Journal Info:
Nlm Unique ID:  0374672     Medline TA:  Immunology     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  271-4     Citation Subset:  IM    
Affiliation:
University of Iowa, Iowa City, 52242, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD
Antigens, CD28 / analysis*,  immunology
Antigens, CD80 / analysis*,  immunology
Antigens, Differentiation / immunology
Biological Assay
CTLA-4 Antigen
Cell Division / immunology
Cell Line
Dose-Response Relationship, Immunologic
Flow Cytometry
Immunoconjugates*
Immunoglobulins / immunology
Interleukin-2 / immunology*
Mice
Receptors, Interleukin-2 / metabolism
T-Lymphocytes / chemistry,  cytology,  immunology*
Grant Support
ID/Acronym/Agency:
CA55178/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/Antigens, CD28; 0/Antigens, CD80; 0/Antigens, Differentiation; 0/CTLA-4 Antigen; 0/Ctla4 protein, mouse; 0/Immunoconjugates; 0/Immunoglobulins; 0/Interleukin-2; 0/Receptors, Interleukin-2; 7D0YB67S97/abatacept
Comments/Corrections

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