| Expression of blood coagulation factors on monocytes after exposure to TNF-treated endothelium in a novel whole blood model of arterial flow. | |
| | |
MedLine Citation:
|
PMID: 19699743 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Activated blood monocytes are a major source of tissue factor (TF), the principal initiator of blood coagulation. TF can be shed from the monocyte surface in association with microparticles (MPs) and increased numbers of circulating MPs are indicative of poor clinical outcome in a number of inflammatory disorders, including atherosclerosis. The mechanisms coupling inflammation with aberrant TF production/activity remain obscure but the protease-activated receptor (PAR) family has been implicated. We have previously shown (i) that freshly isolated human monocytes express low levels of cell surface PAR-2, (ii) that cell surface PAR-2 is rapidly upregulated from intracellular stores following mechanical stimulation, and (iii) that PAR-2 stimulation results in elevation of intracellular calcium and cytokine release. Here, we have investigated the expression of PAR-2 on monocytes exposed to TNF-activated endothelial cells both under static conditions and in our newly-established model of arterial flow, using diluted whole blood. Monocyte surface PAR-2 expression was upregulated following static exposure to activated EC and with laminar (atheroprotective) arterial flow there was a further increase in monocyte PAR-2 expression. We have also shown under arterial flow conditions that exposure to TNF-stimulated EC resulted in a significant increase in expression of TF on monocytes compared to that on cells exposed to quiescent EC. These data strongly suggest that direct or indirect interactions with inflamed EC can modulate expression of PAR-2 and TF on the monocyte cell surface. |
| | |
Authors:
|
Marion G Macey; Sabine I Wolf; Caroline P D Wheeler-Jones; Charlotte Lawson |
Related Documents
:
|
7351483 - Separation of granulocytes from peripheral blood in a single step using discontinuous d... 17583663 - Bovine blood neutrophil acyloxyacyl hydrolase (aoah) activity during endotoxin and coli... 2352053 - Multiparametric analysis of peripheral blood in the normal pediatric population by flow... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-08-21 |
Journal Detail:
|
Title: Journal of immunological methods Volume: 350 ISSN: 1872-7905 ISO Abbreviation: J. Immunol. Methods Publication Date: 2009 Oct |
Date Detail:
|
Created Date: 2009-10-14 Completed Date: 2009-11-09 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 1305440 Medline TA: J Immunol Methods Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 133-41 Citation Subset: IM |
Affiliation:
|
Department of Haematology, The Royal London Hospital, Pathology and Pharmacy Building, 80, Newark Street, London E1 2ES, United Kingdom. marion.macey@bartsandthelondon.nhs.uk |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Atherosclerosis / immunology, metabolism*, pathology Blood Flow Velocity Calcium / immunology, metabolism Cells, Cultured Endothelium, Vascular / immunology, metabolism*, pathology Female Humans Male Models, Cardiovascular* Monocytes / immunology, metabolism*, pathology Receptor, PAR-2 / biosynthesis, immunology Thromboplastin / biosynthesis*, immunology Tumor Necrosis Factor-alpha Up-Regulation / drug effects*, immunology |
| Grant Support | |
ID/Acronym/Agency:
|
//British Heart Foundation |
| Chemical | |
Reg. No./Substance:
|
0/Receptor, PAR-2; 0/Tumor Necrosis Factor-alpha; 7440-70-2/Calcium; 9035-58-9/Thromboplastin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effect of nestling status and brood size on concentration of corticosterone of free-living kittiwake...
Next Document: Differences in usability of rabbit IgG and chicken IgY after clean-up and impact on gold labelling p...