Document Detail


Expression of biologically active bovine luteinizing hormone in Chinese hamster ovary cells.
MedLine Citation:
PMID:  3864159     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Biologically active bovine luteinizing hormone (LH) has been obtained through expression of the alpha- and LH beta-subunit genes in stably transformed clones of DUXB11, a Chinese hamster ovary cell line deficient in dihydrofolate reductase (DHFR). Expression of alpha-and LH beta-subunit mRNAs of the expected sizes (approximately 910 and 770 nucleotides, respectively) were revealed by blot analysis after electrophoresis of total cellular RNA. Furthermore, presence or absence of the gonadotropin mRNAs in several clonal lines was directly correlated with the appearance of one or both bovine LH subunits in the culture medium. Media from three clones secreting significant immunoreactive levels of both subunits also stimulated the release of progesterone in ovine luteal cells, suggesting that the secreted LH was assembled into a biologically active and glycosylated dimer. Immunoprecipitation and NaDodSO4/PAGE of [35S]methionine-labeled proteins secreted from one of the clones, CHODLH20, further confirmed the presence of an alpha/beta dimer with apparent subunit molecular weights of 20,500 and 16,000, only slightly higher than those of pituitary alpha and LH beta subunits.
Authors:
D M Kaetzel; J K Browne; F Wondisford; T M Nett; A R Thomason; J H Nilson
Related Documents :
19589909 - The beta104-109 sequence is essential for the secretion of correctly folded single-chai...
9299349 - Functional cooperation of the mitochondrial processing peptidase subunits.
8043279 - A sequence in the main cytoplasmic loop of the alpha subunit is required for assembly o...
25404739 - The first crystal structure of a dttp bound deoxycytidylate deaminase validates and det...
3108879 - Distinct forms of the beta subunit of gtp-binding regulatory proteins identified by mol...
9325259 - Expression and circular dichroism studies of the extracellular domain of the alpha subu...
16529959 - Phospholipid surfaces regulate the delivery of substrate to tissue factor:viia and the ...
2890329 - The stereoselectivity of the clostridium perfringens phospholipase c: hydrolysis of thi...
10706589 - Interaction of locust apolipophorin iii with lipoproteins and phospholipid vesicles: ef...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  82     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1985 Nov 
Date Detail:
Created Date:  1985-12-05     Completed Date:  1985-12-05     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7280-3     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Cell Line
Corpus Luteum / drug effects,  secretion
Cricetinae
Cricetulus
DNA, Recombinant / metabolism*
Female
Fibroblasts / metabolism
Genetic Vectors
Luteinizing Hormone / biosynthesis*,  genetics,  pharmacology
Ovary
Progesterone / secretion
Recombinant Proteins / genetics,  metabolism*,  pharmacology
Sheep
Grant Support
ID/Acronym/Agency:
AM-01316/AM/NIADDK NIH HHS; AM-06981/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Recombinant; 0/Recombinant Proteins; 57-83-0/Progesterone; 9002-67-9/Luteinizing Hormone
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Photoaffinity labeling of diphtheria toxin fragment A with NAD: structure of the photoproduct at pos...
Next Document:  Molecular cloning and nucleotide sequence of human glucocerebrosidase cDNA.