Document Detail

Expression of bcl-2 and 3-beta hydroxysteroid dehydrogenase protein during oocyte and follicle development in fetal and post-natal pig ovaries.
MedLine Citation:
PMID:  11101284     Owner:  NLM     Status:  MEDLINE    
The fetal and post-natal development of the pig ovary involves both proliferation and apoptotic loss of germ cells, follicle formation and growth, and the initiation of oocyte meiotic maturation. The present study measured the expression of the proto-oncogene Bcl-2 immunohistochemically on paraffin sections of pig ovaries to determine its relationship with folliculogenesis on Days 50 and 80 post coitum (p.c.) and on Days 1, 21, and 56 post partum (p.p.). The expression of the steroidogenic enzyme 3beta-hydroxysteroid dehydrogenase (3betaHSD) was used to determine the lineages of the cells forming the ovarian follicles, and the expression of the cell proliferation-associated nuclear antigen Ki-67 was used to determine germ cell proliferation and the initiation of follicle growth. Expression of Ki-67 showed that many oogonia were proliferating on Days 50 and 80 p.c. Granulosa cells were more proliferative on Day 56 p.p. than at any other stage; Ki-67 was expressed in 70% of growing follicles and granulosa cells had a 3% mean staining index per section. Less than 4% of germ cells and follicles had morphological signs of degeneration during the period of the study. Bcl-2 was most abundant on Days 21 p.p. and 56 p.p.; staining was localized to stromal cells among follicles and in small clusters in the cortical medullary junction (CMJ). 3BetaHSD staining on Day 50 p.c. was seen in cords of stromal cells within the medulla of the ovary, and in the stromal cells investing the oogonial nests. On Days 80 p.c., 1 p.p., 21 p.p., and 56 p.p., 3betaHSD was expressed in the granulosa cells of primary or primordial follicles at the CMJ. Production of Bcl-2 by somatic cells may support germ cell and preantral follicle survival.
W M Garrett; H D Guthrie
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Reproduction, fertility, and development     Volume:  11     ISSN:  1031-3613     ISO Abbreviation:  Reprod. Fertil. Dev.     Publication Date:  1999  
Date Detail:
Created Date:  2000-12-06     Completed Date:  2000-12-22     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8907465     Medline TA:  Reprod Fertil Dev     Country:  AUSTRALIA    
Other Details:
Languages:  eng     Pagination:  463-70     Citation Subset:  IM    
Germplasm and Gamete Physiology Laboratory, Agricultural Research Service, USDA, Beltsville, MD 20705, USA.
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MeSH Terms
3-Hydroxysteroid Dehydrogenases / genetics*
Cell Nucleus / ultrastructure
Cytoplasm / ultrastructure
Gene Expression
Ki-67 Antigen / analysis
Oocytes / physiology*,  ultrastructure
Ovarian Follicle / growth & development*
Ovary / embryology,  growth & development*,  metabolism
Proto-Oncogene Proteins c-bcl-2 / genetics*
Stromal Cells
Reg. No./Substance:
0/Ki-67 Antigen; 0/Proto-Oncogene Proteins c-bcl-2; EC 1.1.-/3-Hydroxysteroid Dehydrogenases

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