Document Detail


Expression of basal cell marker revealed by RAM11 antibody during epithelial regeneration in rabbits.
MedLine Citation:
PMID:  20529822     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
RAM11 is a mouse monoclonal anti-rabbit macrophage antibody recognizing connective tissue and vascular macrophages. Our previous report showed that RAM11 reacted with basal cells of stratified squamous epithelia of rabbit skin, oral mucosa and esophagus. The aim of the present study was to follow the appearance of RAM11 immunoreactivity in basal cells of regenerating oral epithelium in rabbits. No RAM11 immunostaining was observed in the regenerating epithelium examined on days 1 and 3 of wound healing. A weak immunofluorescence first appeared on day 7 in single basal cells and 32% of RAM11- positive basal cells were observed on day 14. These findings indicate that expression of the antigen recognized by RAM11 antibody is a transient event in the differentiation of oral keratinocytes which not always occurs during epithelial repair, although it is a constant feature of epithelial turnover in mature epithelium. Therefore this antigen can be regarded as basal cell marker only in mature stratified squamous epithelia.
Authors:
Grzegorz J Lis; Ewa Jasek; Jan A Litwin; Mariusz Gajda; Joanna Zarzecka; Tadeusz Cichocki
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society     Volume:  48     ISSN:  1897-5631     ISO Abbreviation:  Folia Histochem. Cytobiol.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2010-06-10     Completed Date:  2010-09-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8502651     Medline TA:  Folia Histochem Cytobiol     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  89-92     Citation Subset:  IM    
Affiliation:
Department of Histology, Institute of Stomatology, Jagiellonian University Medical College, Kraków, Poland. mmlis@cyf-kr.edu.pl
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / immunology*
Biological Markers / metabolism
Epithelial Cells / cytology,  metabolism
Epithelium / physiology*
Microscopy, Fluorescence
Rabbits
Regeneration / physiology*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Biological Markers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Vitrification vs. slow cooling protocol using embryos cryopreserved in the 5th or 6th day after oocy...
Next Document:  The mRNA expression of pro- and anti-inflammatory cytokines in T regulatory cells in children with t...