Document Detail

Expression of a bacterial bi-functional chorismate mutase/prephenate dehydratase modulates primary and secondary metabolism associated with aromatic amino acids in Arabidopsis.
MedLine Citation:
PMID:  19508381     Owner:  NLM     Status:  MEDLINE    
Plants can synthesize the aromatic amino acid Phe via arogenate, but it is still not known whether they also use an alternative route for Phe biosynthesis via phenylpyruvate, like many micro-organisms. To examine this possibility, we expressed a bacterial bi-functional PheA (chorismate mutase/prephenate dehydratase) gene in Arabidopsis thaliana that converts chorismate via prephenate into phenylpyruvate. The PheA-expressing plants showed a large increase in the level of Phe, implying that they can convert phenylpyruvate into Phe. In addition, PheA expression rendered the plants more sensitive than wild-type plants to the Trp biosynthesis inhibitor 5-methyl-Trp, implying that Phe biosynthesis competes with Trp biosynthesis from their common precursor chorismate. Surprisingly, GC-MS, LC-MS and microarray analyses showed that this increase in Phe accumulation only had a very minor effect on the levels of other primary metabolites as well as on the transcriptome profile, implying little regulatory cross-interaction between the aromatic amino acid biosynthesis network and the bulk of the Arabidopsis transcriptome and primary metabolism. However, the levels of a number of secondary metabolites derived from all three aromatic amino acids (Phe, Trp and Tyr) were altered in the PheA plants, implying regulatory cross-interactions between the flux of aromatic amino acid biosynthesis from chorismate and their further metabolism into various secondary metabolites. Taken together, our results provide insights into the regulatory mechanisms of aromatic amino acid biosynthesis and their interaction with central primary metabolism, as well as the regulatory interface between primary and secondary metabolism.
Vered Tzin; Sergey Malitsky; Asaph Aharoni; Gad Galili
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-06-06
Journal Detail:
Title:  The Plant journal : for cell and molecular biology     Volume:  60     ISSN:  1365-313X     ISO Abbreviation:  Plant J.     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-10-02     Completed Date:  2009-11-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9207397     Medline TA:  Plant J     Country:  England    
Other Details:
Languages:  eng     Pagination:  156-67     Citation Subset:  IM    
Department of Plant Sciences, The Weizmann Institute of Science, Rehovot 76100, Israel.
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MeSH Terms
Amino Acids, Aromatic / metabolism*
Arabidopsis / genetics,  metabolism*
Chorismic Acid / metabolism
Computational Biology
Escherichia coli / enzymology,  genetics
Escherichia coli Proteins / genetics,  metabolism
Gene Expression Regulation, Plant
Multienzyme Complexes / genetics,  metabolism
Oligonucleotide Array Sequence Analysis
Phenylalanine / biosynthesis
Plants, Genetically Modified / genetics,  metabolism
Prephenate Dehydratase / genetics*,  metabolism
RNA, Plant / genetics
Reg. No./Substance:
0/Amino Acids, Aromatic; 0/Escherichia coli Proteins; 0/Multienzyme Complexes; 0/P-protein, E coli; 0/RNA, Plant; 617-12-9/Chorismic Acid; 63-91-2/Phenylalanine; EC Dehydratase

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