Document Detail

Expression of arachidonate 12-lipoxygenase in rat tissues: a possible role in glucagon secretion.
MedLine Citation:
PMID:  10990494     Owner:  NLM     Status:  MEDLINE    
There are three isoforms of arachidonate 12-lipoxygenase in mammals: platelet, leukocyte, and epidermal types. We found in this study that the leukocyte-type enzyme was present in rat pineal gland, lung, spleen, aorta, adrenal gland, spinal cord, and pancreas, as assessed by RT-PCR. Immunohistochemical analysis showed that the enzyme was localized in macrophages in lung and spleen, alpha-cells of pancreatic islet, zona glomerulosa cells of adrenal cortex, and neuronal cells of spinal cord and superior cervical ganglion. The presence of the 12-lipoxygenase in pancreatic alpha-cells was confirmed by glucagon staining in a consecutive section. We overexpressed the leukocyte-type 12-lipoxygenase cDNA in a glucagon-secreting alphaTC clone 6 cell line that had been established from a transgenic mouse. Glucagon secretion was stimulated by approximately twofold in the 12-lipoxygenase-expressing cells compared to the mock-transfected and original cells. The results suggest that the 12-lipoxygenase of the leukocyte type augments glucagon secretion from pancreatic islets.
H Kawajiri; D Zhuang; N Qiao; T Yoshimoto; M Yamamoto; S Iseki; K Hamaguchi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  48     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  2000 Oct 
Date Detail:
Created Date:  2000-10-10     Completed Date:  2000-10-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1411-9     Citation Subset:  IM    
Department of Pharmacology, Kanazawa University School of Medicine, Kanazawa, Japan.
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MeSH Terms
Antibody Specificity
Arachidonate 12-Lipoxygenase / immunology,  metabolism*
Blotting, Western
Cell Line
Glucagon / secretion*
Islets of Langerhans / metabolism
Leukocytes / enzymology
Macrophages / metabolism
Mice, Transgenic
Organ Specificity
Pineal Gland / enzymology
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Reg. No./Substance:
0/RNA, Messenger; 9007-92-5/Glucagon; EC 12-Lipoxygenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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