| Expression of aquaporins in the retina of diabetic rats. | |
| | |
MedLine Citation:
|
PMID: 21851171 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Purpose/Aim: The development of retinal edema is the main reason of impaired vision in non-proliferative diabetic retinopathy. Water transport through aquaporins (AQPs) has been suggested to facilitate the development of ischemic edema in the retina. Here, we investigated whether experimental diabetic retinopathy in rats results in alterations of the AQP expression in the neural retina and retinal pigment epithelium (RPE). Materials and Methods: Experimental diabetes in rats was induced by a single intravenous injection of streptozotocin (65 mg/kg body weight). The gene expression of AQPs in tissues from control and diabetic rats was examined by real-time RT-PCR. Retinal cryosections were immunostained against AQP5, 6, and 9. Results: The total RNAs extracted from the neural retina and RPE contained gene transcripts for AQP0, 1, 3, 4, 5, 6, 8, 9, 11, and 12. Experimental diabetes was associated with an upregulation of AQP1 in the neural retina, and of AQP5, 9, 11, and 12 in the RPE. Furthermore, diabetes was associated with a downregulation of AQP6 and AQP11 in the neural retina, and of AQP0 in the RPE. AQP5 and AQP9 immunolabelings of the RPE were increased, and AQP6 labeling of the outer plexiform layer was decreased in retinal slices from diabetic rats in comparison to slices from control rats. Conclusions: The data suggest that experimental diabetic retinopathy is associated with a complex pattern of alteration in the retinal AQP expression. These alterations might be involved in the adaptation of retinal cells to hyperglycemic conditions and the development and/or resolution of retinal edema. |
| | |
Authors:
|
Margrit Hollborn; Sladjana Dukic-Stefanovic; Thomas Pannicke; Elke Ulbricht; Andreas Reichenbach; Peter Wiedemann; Andreas Bringmann; Leon Kohen |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Current eye research Volume: 36 ISSN: 1460-2202 ISO Abbreviation: Curr. Eye Res. Publication Date: 2011 Sep |
Date Detail:
|
Created Date: 2011-08-19 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8104312 Medline TA: Curr Eye Res Country: England |
Other Details:
|
Languages: eng Pagination: 850-6 Citation Subset: IM |
Affiliation:
|
Department of Ophthalmology and Eye Hospital, University of Leipzig , Leipzig , Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Hypoxia Specific SDF-1 Expression by Retinal Pigment Epithelium Initiates Bone Marrow-derived Cells ...
Next Document: Genetic variations in the interleukin-6 and interleukin-8 genes and the interleukin-6 receptor gene ...