| Expression of antimicrobial peptides in diabetic foot ulcer. | |
| | |
MedLine Citation:
|
PMID: 22047630 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
BACKGROUND: Foot ulcers are one of the main diabetes complications due to its high frequency and difficulty of complete healing. There are several factors that participate in diabetic ulcers development and limited information exists about the role of antimicrobial peptides (AMP) in its pathogenesis. OBJECTIVE: The aim of this study was to analyze the expression pattern of the main AMPs: Human Neutrophil Peptide (HNP)-1, Human β-defensin (HBD)-1, HBD-2, HBD-3, HBD-4 and cathelicidin LL-37 in biopsies from diabetic foot ulcers (DFU). METHODS: 20 biopsies from DFU grade 3 according to Wagner's classification and 20 biopsies from healthy donors were obtained. Real time PCR, immunohistochemistry and primary cell cultures were performed. RESULTS: β-Defensins were overexpressed in DFU, whereas LL-37 has low or none expression in comparison with healthy skin. When primary cell culture from these biopsies were performed and infected with Staphylococcus aureus, epidermal cell from diabetic ulcers showed lower LL-37 expression compared with cell cultures from healthy donors skin. CONCLUSION: These results suggest that though most AMPs are expressed in DFU, this production is not appropriate to promote wound healing and contain secondary infections. |
| | |
Authors:
|
Bruno Rivas-Santiago; Valentín Trujillo; Alejandra Montoya; Irma Gonzalez-Curiel; Julio Castañeda-Delgado; Albertina Cardenas; Kublai Rincon; Maria L Hernandez; Rogelio Hernández-Pando |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-10-17 |
Journal Detail:
|
Title: Journal of dermatological science Volume: - ISSN: 1873-569X ISO Abbreviation: - Publication Date: 2011 Oct |
Date Detail:
|
Created Date: 2011-11-3 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9011485 Medline TA: J Dermatol Sci Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011. Published by Elsevier Ireland Ltd. |
Affiliation:
|
Medical Research Unit-Zacatecas, Mexican Institute of Social Security (IMSS), Zacatecas, Mexico. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Polypeptide Folding-Mediated Tuning of the Optical and Structural Properties of Gold Nanoparticle As...
Next Document: Standardization of preanalytical aspects of cerebrospinal fluid biomarker testing for Alzheimer's di...